دورية أكاديمية
Rapid identification of bio-molecules applied for detection of biosecurity agents using rolling circle amplification.
| العنوان: | Rapid identification of bio-molecules applied for detection of biosecurity agents using rolling circle amplification. |
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| المؤلفون: | Jenny Göransson, Rongqin Ke, Rachel Yuan Nong, W Mathias Howell, Anna Karman, Jan Grawé, Johan Stenberg, Malin Granberg, Magnus Elgh, David Herthnek, Per Wikström, Jonas Jarvius, Mats Nilsson |
| المصدر: | PLoS ONE, Vol 7, Iss 2, p e31068 (2012) |
| بيانات النشر: | Public Library of Science (PLoS), 2012. |
| سنة النشر: | 2012 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | Detection and identification of pathogens in environmental samples for biosecurity applications are challenging due to the strict requirements on specificity, sensitivity and time. We have developed a concept for quick, specific and sensitive pathogen identification in environmental samples. Target identification is realized by padlock- and proximity probing, and reacted probes are amplified by RCA (rolling-circle amplification). The individual RCA products are labeled by fluorescence and enumerated by an instrument, developed for sensitive and rapid digital analysis. The concept is demonstrated by identification of simili biowarfare agents for bacteria (Escherichia coli and Pantoea agglomerans) and spores (Bacillus atrophaeus) released in field. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1932-6203 |
| Relation: | http://europepmc.org/articles/PMC3285169?pdf=render; https://doaj.org/toc/1932-6203 |
| DOI: | 10.1371/journal.pone.0031068 |
| URL الوصول: | https://doaj.org/article/0f3076f9b3dd4b21867f97a44bd4149f |
| رقم الأكسشن: | edsdoj.0f3076f9b3dd4b21867f97a44bd4149f |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0031068 |