دورية أكاديمية
Omics analysis revealed the intestinal toxicity induced by aflatoxin B1 and aflatoxin M1
| العنوان: | Omics analysis revealed the intestinal toxicity induced by aflatoxin B1 and aflatoxin M1 |
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| المؤلفون: | Ya-Nan Gao, Zi-Wei Wang, Chuan-You Su, Jia-Qi Wang, Nan Zheng |
| المصدر: | Ecotoxicology and Environmental Safety, Vol 278, Iss , Pp 116336- (2024) |
| بيانات النشر: | Elsevier, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Environmental pollution LCC:Environmental sciences |
| مصطلحات موضوعية: | Aflatoxin B1, Aflatoxin M1, Intestinal toxicity, Proteomics, Co-exposure, Environmental pollution, TD172-193.5, Environmental sciences, GE1-350 |
| الوصف: | Aflatoxin B1 (AFB1), a common mycotoxin, can occur in agricultural products. As a metabolite of AFB1, aflatoxin M1 (AFM1) mainly exist in dairy products. These two mycotoxins threaten human health, although it is unclear how they affect the function of the intestinal barrier. In this study, mice were exposed to AFB1 (0.3 mg/kg body b.w.) and AFM1(3.0 mg/kg b.w.) either individually or in combination for 28 days to explore the main differentially expressed proteins (DEPs) and the associated enriched pathways. These findings were preliminarily verified by the transcriptomic and proteomic analyses in differentiated Caco-2 cells. The results revealed that AFB1 and AFM1 exposure in mice disrupted the function of the intestinal barrier, and the combined toxicity was greater than that of each toxin alone. Further proteomic analysis in mice demonstrated that the mechanisms underlying these differences could be explained as follows: (i) lipid metabolism was enriched by AFB1-induced DEPs. (ii) protein export pathway was stimulated by AFM1-induced DEPs. (iii) cell metabolic ability was inhibited (as evidenced by changes in UDP-GT1, UDP-GT2, and Gatm6), apoptosis was induced (MAP4K3), and epithelial cell integrity was disrupted (Claudin7 and IQGAP2), resulting in more extensive intestinal damage after combined treatment. In conclusion, the hazardous impact of co-exposure to AFB1 and AFM1 from proteomic perspectives was demonstrated in the present study. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0147-6513 93534930 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S0147651324004123; https://doaj.org/toc/0147-6513 |
| DOI: | 10.1016/j.ecoenv.2024.116336 |
| URL الوصول: | https://doaj.org/article/0f594cef548c4f0bbd106e93534930fd |
| رقم الأكسشن: | edsdoj.0f594cef548c4f0bbd106e93534930fd |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 01476513 93534930 |
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| DOI: | 10.1016/j.ecoenv.2024.116336 |