دورية أكاديمية
Reducing Immunoreactivity of Gluten Peptides by Probiotic Lactic Acid Bacteria for Dietary Management of Gluten-Related Diseases
| العنوان: | Reducing Immunoreactivity of Gluten Peptides by Probiotic Lactic Acid Bacteria for Dietary Management of Gluten-Related Diseases |
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| المؤلفون: | Joanna Leszczyńska, Agnieszka K. Szczepankowska, Iwona Majak, Dorota Mańkowska, Beata Smolińska, Sylwia Ścieszka, Anna Diowksz, Bożena Cukrowska, Tamara Aleksandrzak-Piekarczyk |
| المصدر: | Nutrients, Vol 16, Iss 7, p 976 (2024) |
| بيانات النشر: | MDPI AG, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Nutrition. Foods and food supply |
| مصطلحات موضوعية: | celiac disease, gluten-related diseases, gluten-free diet, endopeptidase, 33-mer peptide, peptidase-encoding genes, Nutrition. Foods and food supply, TX341-641 |
| الوصف: | Immunoreactive gluten peptides that are not digested by peptidases produced by humans can trigger celiac disease, allergy and non-celiac gluten hypersensitivity. The aim of this study was to evaluate the ability of selected probiotic strains to hydrolyze immunoreactive gliadin peptides and to identify peptidase-encoding genes in the genomes of the most efficient strains. Residual gliadin immunoreactivity was measured after one- or two-step hydrolysis using commercial enzymes and bacterial peptidase preparations by G12 and R5 immunoenzymatic assays. Peptidase preparations from Lacticaseibacillus casei LC130, Lacticaseibacillus paracasei LPC100 and Streptococcus thermophilus ST250 strains significantly reduced the immunoreactivity of gliadin peptides, including 33-mer, and this effect was markedly higher when a mixture of these strains was used. In silico genome analyses of L. casei LC130 and L. paracasei LPC100 revealed the presence of genes encoding peptidases with the potential to hydrolyze bonds in proline-rich peptides. This suggests that L. casei LC130, L. paracasei LPC100 and S. thermophilus ST250, especially when used as a mixture, have the ability to hydrolyze immunoreactive gliadin peptides and could be administered to patients on a restricted gluten-free diet to help treat gluten-related diseases. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 16070976 2072-6643 |
| Relation: | https://www.mdpi.com/2072-6643/16/7/976; https://doaj.org/toc/2072-6643 |
| DOI: | 10.3390/nu16070976 |
| URL الوصول: | https://doaj.org/article/14919ed799614b9ba44232c138ecd36f |
| رقم الأكسشن: | edsdoj.14919ed799614b9ba44232c138ecd36f |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 16070976 20726643 |
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| DOI: | 10.3390/nu16070976 |