دورية أكاديمية
Development of buffalo (Bubalus bubalis) embryonic stem cell lines from somatic cell nuclear transferred blastocysts
| العنوان: | Development of buffalo (Bubalus bubalis) embryonic stem cell lines from somatic cell nuclear transferred blastocysts |
|---|---|
| المؤلفون: | Syed Mohmad Shah, Neha Saini, Syma Ashraf, Manoj K Singh, Radheysham Manik, Suresh K Singla, Prabhat Palta, Manmohan Singh Chauhan |
| المصدر: | Stem Cell Research, Vol 15, Iss 3, Pp 633-639 (2015) |
| بيانات النشر: | Elsevier, 2015. |
| سنة النشر: | 2015 |
| المجموعة: | LCC:Biology (General) |
| مصطلحات موضوعية: | Biology (General), QH301-705.5 |
| الوصف: | We developed buffalo embryonic stem cell lines from somatic cell nuclear transfer derived blastocysts, produced by hand-guided cloning technique. The inner cell mass of the blastocyst was cut mechanically using a Microblade and cultured onto feeder cells in buffalo embryonic stem (ES) cell culture medium at 38 °C in a 5% CO2 incubator. The stem cell colonies were characterized for alkaline phosphatase activity, karyotype, pluripotency and self-renewal markers like OCT4, NANOG, SOX2, c-Myc, FOXD3, SSEA-1, SSEA-4, TRA-1-60, TRA-1-81 and CD90. The cell lines also possessed the capability to differentiate across all the three germ layers under spontaneous differentiation conditions. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1873-5061 1876-7753 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S1873506115001452; https://doaj.org/toc/1873-5061; https://doaj.org/toc/1876-7753 |
| DOI: | 10.1016/j.scr.2015.10.010 |
| URL الوصول: | https://doaj.org/article/14e020e5a82f480d9411bbb05cc14f59 |
| رقم الأكسشن: | edsdoj.14e020e5a82f480d9411bbb05cc14f59 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 18735061 18767753 |
|---|---|
| DOI: | 10.1016/j.scr.2015.10.010 |