A simple ATAC-seq protocol for population epigenetics [version 2; peer review: 2 approved]

التفاصيل البيبلوغرافية
العنوان:	A simple ATAC-seq protocol for population epigenetics [version 2; peer review: 2 approved]
المؤلفون:	Ronaldo de Carvalho Augusto, Oliver Rey, Céline Cosseau, Cristian Chaparro, Jérémie Vidal-Dupiol, Jean-François Allienne, David Duval, Silvain Pinaud, Sina Tönges, Ranja Andriantsoa, Emilien Luquet, Fabien Aubret, Mamadou Dia Sow, Patrice David, Vicki Thomson, Dominique Joly, Mariana Gomes Lima, Déborah Federico, Etienne Danchin, Aki Minoda, Christoph Grunau
المصدر:	Wellcome Open Research, Vol 5 (2021)
بيانات النشر:	Wellcome, 2021.
سنة النشر:	2021
المجموعة:	LCC:Medicine LCC:Science
مصطلحات موضوعية:	Medicine, Science
الوصف:	We describe here a protocol for the generation of sequence-ready libraries for population epigenomics studies, and the analysis of alignment results. We show that the protocol can be used to monitor chromatin structure changes in populations when exposed to environmental cues. The protocol is a streamlined version of the Assay for transposase accessible chromatin with high-throughput sequencing (ATAC-seq) that provides a positive display of accessible, presumably euchromatic regions. The protocol is straightforward and can be used with small individuals such as daphnia and schistosome worms, and probably many other biological samples of comparable size (~10,000 cells), and it requires little molecular biology handling expertise.
نوع الوثيقة:	article
وصف الملف:	electronic resource
اللغة:	English
تدمد:	2398-502X
Relation:	https://wellcomeopenresearch.org/articles/5-121/v2; https://doaj.org/toc/2398-502X
DOI:	10.12688/wellcomeopenres.15552.2
URL الوصول:	https://doaj.org/article/15891f8e89f645118d4b376dd9e6a6ad
رقم الأكسشن:	edsdoj.15891f8e89f645118d4b376dd9e6a6ad
قاعدة البيانات:	Directory of Open Access Journals

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تدمد:	2398502X
DOI:	10.12688/wellcomeopenres.15552.2