دورية أكاديمية
RhoA improves cryopreservation of rooster sperm through the Rho/RhoA-associated kinase/cofilin pathway
| العنوان: | RhoA improves cryopreservation of rooster sperm through the Rho/RhoA-associated kinase/cofilin pathway |
|---|---|
| المؤلفون: | Nuo Heng, Zhi-Xian Zhao, Yong Guo, Shan Gao, De-Lin Cai, Bo-Fan Fu, Xi-Hui Sheng, Xiang-Guo Wang, Kai Xing, Long-Fei Xiao, Cheng Long, He-Min Ni, Hua-Bin Zhu, Xiao-Long Qi |
| المصدر: | Poultry Science, Vol 101, Iss 10, Pp 102051- (2022) |
| بيانات النشر: | Elsevier, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Animal culture |
| مصطلحات موضوعية: | RhoA, rooster sperm, semen quality, programmed freezing, ultrastructure, cryopreservation, Animal culture, SF1-1100 |
| الوصف: | ABSTRACT: Cryopreservation of rooster sperm leads to relatively low semen quality due to cytoskeletal damage during the freeze-thawing process. This study aimed to explore how the addition of RhoA recombinant protein affected the viability and subcellular structure of rooster sperm after freeze-thawing and elucidated the molecular mechanisms of sperm cryopreservation. Semen quality and acrosome integrity testing revealed that the addition of 0.5 μg/mL RhoA recombinant protein to the cryoprotectant fluid significantly increased sperm motility, survival rate, linearity, straight-line velocity, and acrosome integrity after freeze-thawing (P < 0.05). Ultrastructure analysis of cryopreserved sperm showed structural damage to the sperm plasma membrane, nuclear membrane, and tail. However, compared to the control, these structural changes were reduced upon the addition of RhoA recombinant protein to the cryoprotective fluid (P < 0.05). Western blotting revealed that the expression of Rho/RhoA-associated kinase and p-cofilin was increased, and cofilin expression was decreased after sperm cryopreservation with recombinant RhoA protein. Treatment with Y-27632, a ROCK antagonist, suppressed ROCK and p-cofilin expression and decreased semen quality, acrosome integrity, and ultrastructure integrity. In summary, we have demonstrated a cryoprotective effect in spermatozoa involving the Rho/ROCK pathway during freeze-thawing. Furthermore, the addition of 0.5 μg/mL RhoA recombinant protein to the cryoprotective fluid improved rooster semen quality and subcellular structural homeostasis after freeze-thawing via the Rho/ROCK pathway. This pathway may regulate the dynamic reorganization of the actin cytoskeleton by regulating the cofilin phosphorylation. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0032-5791 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S003257912200342X; https://doaj.org/toc/0032-5791 |
| DOI: | 10.1016/j.psj.2022.102051 |
| URL الوصول: | https://doaj.org/article/aa17a8fd18c3499b80e747098ac6ecbb |
| رقم الأكسشن: | edsdoj.17a8fd18c3499b80e747098ac6ecbb |
| قاعدة البيانات: | Directory of Open Access Journals |
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