دورية أكاديمية
Efficient secretory expression of human milk Osteopontin in Komagataella phaffii
| العنوان: | Efficient secretory expression of human milk Osteopontin in Komagataella phaffii |
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| المؤلفون: | Zhihang Zhang, Yangyang Li, Zhenmin Liu, Shixiu Cui, Xianhao Xu, Yanfeng Liu, Jianghua Li, Guocheng Du, Xueqin Lv, Long Liu |
| المصدر: | Future Foods, Vol 10, Iss , Pp 100393- (2024) |
| بيانات النشر: | Elsevier, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Nutrition. Foods and food supply LCC:Food processing and manufacture |
| مصطلحات موضوعية: | Komagataella phaffii, Osteopontin, Hybrid signal peptide, Molecular chaperone, Secretory expression, Nutrition. Foods and food supply, TX341-641, Food processing and manufacture, TP368-456 |
| الوصف: | Osteopontin (OPN), a bioactive milk protein in breast milk, has diverse applications in food and medicine. OPN is predominantly extracted from fresh bovine milk; however, the concentration of OPN in bovine milk is exceedingly low. To address these issues, in the present study, we used Komagataella phaffii for the recombinant expression of human OPN (hOPN). The results of host cell screening revealed that X33 cells were more suitable than other K. phaffii hosts for hOPN expression, yielding a titer of 340.5 μg/L. Subsequently, it was observed that the extracellular production of hOPN significantly increased to 8.02 mg/L from strain XPSA01 using the PAOX1 promoter and the hybrid signal peptide PROSCW10-α. Meanwhile, the co-expression of transcription factors, translation factors, and molecular chaperones resulted in a 2.94-fold enhancement in extracellular hOPN production compared with that in XPSA01, resulting in a titer of 23.6 mg/L. Finally, the above strategies were combined to obtain the recombinant strain XPSA01-CP, which achieved titers of 35.6 and 128.5 mg/L hOPN in shake-flask fermentation and 3-L bioreactor, respectively. To the best of our knowledge, this is the highest extracellular yield of hOPN ever reported. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2666-8335 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2666833524000996; https://doaj.org/toc/2666-8335 |
| DOI: | 10.1016/j.fufo.2024.100393 |
| URL الوصول: | https://doaj.org/article/d17ef2d75ec147b6a1dc82cd160e122d |
| رقم الأكسشن: | edsdoj.17ef2d75ec147b6a1dc82cd160e122d |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 26668335 |
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| DOI: | 10.1016/j.fufo.2024.100393 |