دورية أكاديمية
Dye-enhanced visualization of rat whiskers for behavioral studies
| العنوان: | Dye-enhanced visualization of rat whiskers for behavioral studies |
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| المؤلفون: | Jacopo Rigosa, Alessandro Lucantonio, Giovanni Noselli, Arash Fassihi, Erik Zorzin, Fabrizio Manzino, Francesca Pulecchi, Mathew E Diamond |
| المصدر: | eLife, Vol 6 (2017) |
| بيانات النشر: | eLife Sciences Publications Ltd, 2017. |
| سنة النشر: | 2017 |
| المجموعة: | LCC:Medicine LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | whisker, beam dynamics, tracking, fluorescence, kinematics, imaging, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | Visualization and tracking of the facial whiskers is required in an increasing number of rodent studies. Although many approaches have been employed, only high-speed videography has proven adequate for measuring whisker motion and deformation during interaction with an object. However, whisker visualization and tracking is challenging for multiple reasons, primary among them the low contrast of the whisker against its background. Here, we demonstrate a fluorescent dye method suitable for visualization of one or more rat whiskers. The process makes the dyed whisker(s) easily visible against a dark background. The coloring does not influence the behavioral performance of rats trained on a vibrissal vibrotactile discrimination task, nor does it affect the whiskers’ mechanical properties. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2050-084X |
| Relation: | https://elifesciences.org/articles/25290; https://doaj.org/toc/2050-084X |
| DOI: | 10.7554/eLife.25290 |
| URL الوصول: | https://doaj.org/article/18f2b5936f5f47f588baa729af1170a4 |
| رقم الأكسشن: | edsdoj.18f2b5936f5f47f588baa729af1170a4 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2050084X |
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| DOI: | 10.7554/eLife.25290 |