دورية أكاديمية
Phospholipase C-γ as a Potential Therapeutic Target for Graves’ Orbitopathy
| العنوان: | Phospholipase C-γ as a Potential Therapeutic Target for Graves’ Orbitopathy |
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| المؤلفون: | Tae Hoon Roh, Min Kyung Chae, Jae Sang Ko, Don O. Kikkawa, Sun Young Jang, Jin Sook Yoon |
| المصدر: | Endocrinology and Metabolism, Vol 38, Iss 6, Pp 739-749 (2023) |
| بيانات النشر: | Korean Endocrine Society, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Diseases of the endocrine glands. Clinical endocrinology |
| مصطلحات موضوعية: | phospholipase c gamma, graves ophthalmopathy, u73122, proinflammatory cytokines, inflammation, orbital fibroblasts, Diseases of the endocrine glands. Clinical endocrinology, RC648-665 |
| الوصف: | Background Phospholipase C-γ (PLC-γ) plays a crucial role in immune responses and is related to the pathogenesis of various inflammatory disorders. In this study, we investigated the role of PLC-γ and the therapeutic effect of the PLC-specific inhibitor U73122 using orbital fibroblasts from patients with Graves’ orbitopathy (GO). Methods The expression of phospholipase C gamma 1 (PLCG1) and phospholipase C gamma 2 (PLCG2) was evaluated using polymerase chain reaction in GO and normal orbital tissues/fibroblasts. The primary cultures of orbital fibroblasts were treated with non-toxic concentrations of U73122 with or without interleukin (IL)-1β to determine its therapeutic efficacy. The proinflammatory cytokine levels and activation of downstream signaling molecules were determined using Western blotting. Results PLCG1 and PLCG2 mRNA expression was significantly higher in GO orbital tissues than in controls (P |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Korean |
| تدمد: | 2093-596X 2093-5978 |
| Relation: | http://www.e-enm.org/upload/pdf/enm-2023-1780.pdf; https://doaj.org/toc/2093-596X; https://doaj.org/toc/2093-5978 |
| DOI: | 10.3803/EnM.2023.1780 |
| URL الوصول: | https://doaj.org/article/d1d6633a0dff4d5280c50faac8b5ec91 |
| رقم الأكسشن: | edsdoj.1d6633a0dff4d5280c50faac8b5ec91 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2093596X 20935978 |
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| DOI: | 10.3803/EnM.2023.1780 |