دورية أكاديمية
Rapid changes in morphogen concentration control self-organized patterning in human embryonic stem cells
| العنوان: | Rapid changes in morphogen concentration control self-organized patterning in human embryonic stem cells |
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| المؤلفون: | Idse Heemskerk, Kari Burt, Matthew Miller, Sapna Chhabra, M Cecilia Guerra, Lizhong Liu, Aryeh Warmflash |
| المصدر: | eLife, Vol 8 (2019) |
| بيانات النشر: | eLife Sciences Publications Ltd, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Medicine LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | nodal, BMP, signaling dynamics, morphogen, self-organization, embryonic stem cells, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | During embryonic development, diffusible signaling molecules called morphogens are thought to determine cell fates in a concentration-dependent way. Yet, in mammalian embryos, concentrations change rapidly compared to the time for making cell fate decisions. Here, we use human embryonic stem cells (hESCs) to address how changing morphogen levels influence differentiation, focusing on how BMP4 and Nodal signaling govern the cell-fate decisions associated with gastrulation. We show that BMP4 response is concentration dependent, but that expression of many Nodal targets depends on rate of concentration change. Moreover, in a self-organized stem cell model for human gastrulation, expression of these genes follows rapid changes in endogenous Nodal signaling. Our study shows a striking contrast between the specific ways ligand dynamics are interpreted by two closely related signaling pathways, highlighting both the subtlety and importance of morphogen dynamics for understanding mammalian embryogenesis and designing optimized protocols for directed stem cell differentiation.Editorial note: This article has been through an editorial process in which the authors decide how to respond to the issues raised during peer review. The Reviewing Editor's assessment is that all the issues have been addressed (see decision letter). |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2050-084X |
| Relation: | https://elifesciences.org/articles/40526; https://doaj.org/toc/2050-084X |
| DOI: | 10.7554/eLife.40526 |
| URL الوصول: | https://doaj.org/article/1e0f3bffc5af49abbb7626d53c395584 |
| رقم الأكسشن: | edsdoj.1e0f3bffc5af49abbb7626d53c395584 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2050084X |
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| DOI: | 10.7554/eLife.40526 |