دورية أكاديمية
Six2 Is a Coordinator of LiCl-Induced Cell Proliferation and Apoptosis
العنوان: | Six2 Is a Coordinator of LiCl-Induced Cell Proliferation and Apoptosis |
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المؤلفون: | Jianing Liu, Pan Ju, Yuru Zhou, Ya Zhao, Yajun Xie, Yaoshui Long, Yuping Gu, Dongsheng Ni, Zhongshi Lyv, Zhaomin Mao, Jin Hao, Yiman Li, Qianya Wan, Quist Kanyomse, Yamin Liu, Yue Xiang, Ruoli Wang, Xiangling Chen, Junman Zhang, Xihan Liu, Hui Zhao, Qin Zhou, Ge Li |
المصدر: | International Journal of Molecular Sciences, Vol 17, Iss 9, p 1504 (2016) |
بيانات النشر: | MDPI AG, 2016. |
سنة النشر: | 2016 |
المجموعة: | LCC:Biology (General) LCC:Chemistry |
مصطلحات موضوعية: | LiCl, metanephric mesenchyme cells, cell proliferation, cell apoptosis, Six2, Biology (General), QH301-705.5, Chemistry, QD1-999 |
الوصف: | The metanephric mesenchyme (MM) cells are a subset of kidney progenitor cells and play an essential role in mesenchymal-epithelial transition (MET), the key step of nephron generation. Six2, a biological marker related to Wnt signaling pathway, promotes the proliferation, inhibits the apoptosis and maintains the un-differentiation of MM cells. Besides, LiCl is an activator of Wnt signaling pathway. However, the role of LiCl in cellular regulation of MM cells remains unclear, and the relationship between LiCl and Six2 in this process is also little known. Here, we performed EdU assay and flow cytometry assay to, respectively, detect the proliferation and apoptosis of MM cells treated with LiCl of increasing dosages. In addition, reverse transcription-PCR (RT-PCR) and Western-blot were conducted to measure the expression of Six2 and some maker genes of Wnt and bone-morphogenetic-protein (BMP) signaling pathway. Furthermore, luciferase assay was also carried out to detect the transcriptional regulation of Six2. Then we found LiCl promoted MM cell proliferation at low-concentration (10, 20, 30, and 40 mM). The expression of Six2 was dose-dependently increased in low-concentration (10, 20, 30, and 40 mM) at both mRNA and protein level. In addition, both of cell proliferation and Six2 expression in MM cells declined when dosage reached high-concentration (50 mM). However, Six2 knock-down converted the proliferation reduction at 50 mM. Furthermore, Six2 deficiency increased the apoptosis of MM cells, compared with negative control cells at relative LiCl concentration. However, the abnormal rise of apoptosis at 30 mM of LiCl concentration implies that it might be the reduction of GSK3β that increased cell apoptosis. Together, these demonstrate that LiCl can induce the proliferation and apoptosis of MM cells coordinating with Six2. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1422-0067 |
Relation: | http://www.mdpi.com/1422-0067/17/9/1504; https://doaj.org/toc/1422-0067 |
DOI: | 10.3390/ijms17091504 |
URL الوصول: | https://doaj.org/article/1ed8e6ac1c204ec2b083117dd78696c7 |
رقم الأكسشن: | edsdoj.1ed8e6ac1c204ec2b083117dd78696c7 |
قاعدة البيانات: | Directory of Open Access Journals |
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