دورية أكاديمية
CRISPR-induced exon skipping is dependent on premature termination codon mutations
| العنوان: | CRISPR-induced exon skipping is dependent on premature termination codon mutations |
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| المؤلفون: | Tingting Sui, Yuning Song, Zhiquan Liu, Mao Chen, Jichao Deng, Yuanyuan Xu, Liangxue Lai, Zhanjun Li |
| المصدر: | Genome Biology, Vol 19, Iss 1, Pp 1-5 (2018) |
| بيانات النشر: | BMC, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Biology (General) LCC:Genetics |
| مصطلحات موضوعية: | Exon skipping, PTC, CRISPR/Cas9, Rabbits, Biology (General), QH301-705.5, Genetics, QH426-470 |
| الوصف: | Abstract In previous studies, CRISPR/Cas9 was shown to induce unexpected exon skipping; however, the mechanism by which this phenomenon is triggered is controversial. By analyzing 22 gene-edited rabbit lines generated using CRISPR/Cas9, we provide evidence of exon skipping at high frequency in premature termination codon-mutated rabbits but not in the rabbits with a premature termination codon mutation in exon 1 rabbits with non-frameshift or missense mutations. Our results suggest that CRISPR-mediated exon skipping depends on premature termination codon mutation-induced nonsense-associated altered splicing. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1474-760X |
| Relation: | http://link.springer.com/article/10.1186/s13059-018-1532-z; https://doaj.org/toc/1474-760X |
| DOI: | 10.1186/s13059-018-1532-z |
| URL الوصول: | https://doaj.org/article/227e3bdccb8e41f8a1375b16c41e6823 |
| رقم الأكسشن: | edsdoj.227e3bdccb8e41f8a1375b16c41e6823 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1474760X |
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| DOI: | 10.1186/s13059-018-1532-z |