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Cryo-EM structure of the mechanically activated ion channel OSCA1.2

التفاصيل البيبلوغرافية
العنوان: Cryo-EM structure of the mechanically activated ion channel OSCA1.2
المؤلفون: Sebastian Jojoa-Cruz, Kei Saotome, Swetha E Murthy, Che Chun Alex Tsui, Mark SP Sansom, Ardem Patapoutian, Andrew B Ward
المصدر: eLife, Vol 7 (2018)
بيانات النشر: eLife Sciences Publications Ltd, 2018.
سنة النشر: 2018
المجموعة: LCC:Medicine
LCC:Science
LCC:Biology (General)
مصطلحات موضوعية: ion channel, electron microscopy, mechanotransduction, Medicine, Science, Biology (General), QH301-705.5
الوصف: Mechanically activated ion channels underlie touch, hearing, shear-stress sensing, and response to turgor pressure. OSCA/TMEM63s are a newly-identified family of eukaryotic mechanically activated ion channels opened by membrane tension. The structural underpinnings of OSCA/TMEM63 function are not explored. Here, we elucidate high resolution cryo-electron microscopy structures of OSCA1.2, revealing a dimeric architecture containing eleven transmembrane helices per subunit and surprising topological similarities to TMEM16 proteins. We locate the ion permeation pathway within each subunit by demonstrating that a conserved acidic residue is a determinant of channel conductance. Molecular dynamics simulations reveal membrane interactions, suggesting the role of lipids in OSCA1.2 gating. These results lay a foundation to decipher how the structural organization of OSCA/TMEM63 is suited for their roles as MA ion channels.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2050-084X
Relation: https://elifesciences.org/articles/41845; https://doaj.org/toc/2050-084X
DOI: 10.7554/eLife.41845
URL الوصول: https://doaj.org/article/23cb84194ec248b0adcd920616c63087
رقم الأكسشن: edsdoj.23cb84194ec248b0adcd920616c63087
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:2050084X
DOI:10.7554/eLife.41845