دورية أكاديمية
Coexpression and Transcriptome analyses identify active Apomixis-related genes in Paspalum notatum leaves
| العنوان: | Coexpression and Transcriptome analyses identify active Apomixis-related genes in Paspalum notatum leaves |
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| المؤلفون: | Fernanda A. de Oliveira, Bianca B. Z. Vigna, Carla C. da Silva, Alessandra P. Fávero, Frederico P. de Matta, Ana L. S. Azevedo, Anete P. de Souza |
| المصدر: | BMC Genomics, Vol 21, Iss 1, Pp 1-15 (2020) |
| بيانات النشر: | BMC, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Biotechnology LCC:Genetics |
| مصطلحات موضوعية: | Differential expression, Gene coexpression network, Apomixis, RNA sequencing, Paspalum, Biotechnology, TP248.13-248.65, Genetics, QH426-470 |
| الوصف: | Abstract Background Paspalum notatum exhibits both sexual and apomictic cytotypes and, thus, is considered a good model for studies of apomixis because it facilitates comparative approaches. In this work, transcriptome sequencing was used to compare contrasting P. notatum cytotypes to identify differential expression patterns and candidate genes involved in the regulation of expression of this trait. Results We built a comprehensive transcriptome using leaf and inflorescence from apomictic tetraploids and sexual diploids/tetraploids and a coexpression network based on pairwise correlations between transcript expression profiles. We identified genes exclusively expressed in each cytotype and genes differentially expressed between pairs of cytotypes. Gene Ontology enrichment analyses were performed to better interpret the data. We de novo assembled 114,306 reference transcripts. In total, 536 candidate genes possibly associated with apomixis were detected through statistical analyses of the differential expression data, and several interacting genes potentially linked to the apomixis-controlling region, genes that have already been reported in the literature, and their neighbors were transcriptionally related in the coexpression network. Conclusions Apomixis is a highly desirable trait in modern agriculture due to the maintenance of the characteristics of the mother plant in the progeny. The reference transcriptome, candidate genes and their coexpression network identified in this work represent rich resources for future grass breeding programs. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1471-2164 |
| Relation: | https://doaj.org/toc/1471-2164 |
| DOI: | 10.1186/s12864-020-6518-z |
| URL الوصول: | https://doaj.org/article/24c44bdc4f3b457d8d538bda95e0929c |
| رقم الأكسشن: | edsdoj.24c44bdc4f3b457d8d538bda95e0929c |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 14712164 |
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| DOI: | 10.1186/s12864-020-6518-z |