دورية أكاديمية
Role of Cathepsin S in Periodontal Inflammation and Infection
العنوان: | Role of Cathepsin S in Periodontal Inflammation and Infection |
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المؤلفون: | S. Memmert, A. Damanaki, A. V. B. Nogueira, S. Eick, M. Nokhbehsaim, A. K. Papadopoulou, A. Till, B. Rath, S. Jepsen, W. Götz, C. Piperi, E. K. Basdra, J. A. Cirelli, A. Jäger, J. Deschner |
المصدر: | Mediators of Inflammation, Vol 2017 (2017) |
بيانات النشر: | Wiley, 2017. |
سنة النشر: | 2017 |
المجموعة: | LCC:Pathology |
مصطلحات موضوعية: | Pathology, RB1-214 |
الوصف: | Cathepsin S is a cysteine protease and regulator of autophagy with possible involvement in periodontitis. The objective of this study was to investigate whether cathepsin S is involved in the pathogenesis of periodontal diseases. Human periodontal fibroblasts were cultured under inflammatory and infectious conditions elicited by interleukin-1β and Fusobacterium nucleatum, respectively. An array-based approach was used to analyze differential expression of autophagy-associated genes. Cathepsin S was upregulated most strongly and thus further studied in vitro at gene and protein levels. In vivo, gingival tissue biopsies from rats with ligature-induced periodontitis and from periodontitis patients were also analyzed at transcriptional and protein levels. Multiple gene expression changes due to interleukin-1β and F. nucleatum were observed in vitro. Both stimulants caused a significant cathepsin S upregulation. A significantly elevated cathepsin S expression in gingival biopsies from rats with experimental periodontitis was found in vivo, as compared to that from control. Gingival biopsies from periodontitis patients showed a significantly higher cathepsin S expression than those from healthy gingiva. Our findings provide original evidence that cathepsin S is increased in periodontal cells and tissues under inflammatory and infectious conditions, suggesting a critical role of this autophagy-associated molecule in the pathogenesis of periodontitis. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 0962-9351 1466-1861 |
Relation: | https://doaj.org/toc/0962-9351; https://doaj.org/toc/1466-1861 |
DOI: | 10.1155/2017/4786170 |
URL الوصول: | https://doaj.org/article/25f310afe6034165a67654f127794968 |
رقم الأكسشن: | edsdoj.25f310afe6034165a67654f127794968 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 09629351 14661861 |
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DOI: | 10.1155/2017/4786170 |