دورية أكاديمية
Conditioned culture medium of bone marrow mesenchymal stem cells promotes phenotypic transformation of microglia by regulating mitochondrial autophagy
العنوان: | Conditioned culture medium of bone marrow mesenchymal stem cells promotes phenotypic transformation of microglia by regulating mitochondrial autophagy |
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المؤلفون: | Hangyu Ji, Weiming Chu, Yong Yang, Xin Peng, Xiaoli Song |
المصدر: | PeerJ, Vol 12, p e17664 (2024) |
بيانات النشر: | PeerJ Inc., 2024. |
سنة النشر: | 2024 |
المجموعة: | LCC:Medicine LCC:Biology (General) |
مصطلحات موضوعية: | Conditioned medium, Bone marrow mesenchymal stem cells, Microglia, Polarization, Mitochondrial autophagy, Medicine, Biology (General), QH301-705.5 |
الوصف: | Objective To study the mechanism by which conditioned medium of bone marrow mesenchymal stem cells (BMSCs-CM) facilitates the transition of pro-inflammatory polarized microglia to an anti-inflammatory phenotype. Methods BV2 cells, a mouse microglia cell line, were transformed into a pro-inflammatory phenotype using lipopolysaccharide. The expression of phenotypic genes in BV2 cells was detected using real-time quantitative PCR (RT-qPCR). Enzyme-linked immunosorbent assay was used to measure inflammatory cytokine levels in BV2 cells co-cultured with BMSCs-CM. The expressions of mitophagy-associated proteins were determined using western blot. The mitochondrial membrane potential and ATP levels in BV2 cells were measured using JC-1 staining and an ATP assay kit, respectively. Additionally, we examined the proliferation, apoptosis, and migration of C8-D1A cells, a mouse astrocyte cell line, co-cultured with BV2 cells. Results After co- culture with BMSCs -CM, the mRNA expression of tumor necrosis factor-α (TNF-α) and inducible nitric oxide synthase significantly decreased in pro-inflammatory BV2 cells, whereas the expression of CD206 and arginase-1 significantly increased. Moreover, TNF-α and interleukin-6 levels significantly decreased, whereas transforming growth factor-β and interleukin-10 levels significantly increased. Furthermore, co-culture with BMSCs-CM increased mitophagy-associated protein expression, ATP levels, mitochondrial and lysosomal co-localization in these cells and decreased reactive oxygen species levels. Importantly, BMSCs-CM reversed the decrease in the proliferation and migration of C8-D1A cells co-cultured with pro-inflammatory BV2 cells and inhibited the apoptosis of C8-D1A cells. Conclusion BMSCs-CM may promote the transition of polarized microglia from a pro-inflammatory to an anti-inflammatory phenotype by regulating mitophagy and influences the functional state of astrocytes. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 2167-8359 |
Relation: | https://peerj.com/articles/17664.pdf; https://peerj.com/articles/17664/; https://doaj.org/toc/2167-8359 |
DOI: | 10.7717/peerj.17664 |
URL الوصول: | https://doaj.org/article/2683e8303b4a4f2ab0e934452a7befd9 |
رقم الأكسشن: | edsdoj.2683e8303b4a4f2ab0e934452a7befd9 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 21678359 |
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DOI: | 10.7717/peerj.17664 |