دورية أكاديمية

Transcriptomic analysis of tail regeneration in the lizard Anolis carolinensis reveals activation of conserved vertebrate developmental and repair mechanisms.

التفاصيل البيبلوغرافية
العنوان: Transcriptomic analysis of tail regeneration in the lizard Anolis carolinensis reveals activation of conserved vertebrate developmental and repair mechanisms.
المؤلفون: Elizabeth D Hutchins, Glenn J Markov, Walter L Eckalbar, Rajani M George, Jesse M King, Minami A Tokuyama, Lauren A Geiger, Nataliya Emmert, Michael J Ammar, April N Allen, Ashley L Siniard, Jason J Corneveaux, Rebecca E Fisher, Juli Wade, Dale F DeNardo, J Alan Rawls, Matthew J Huentelman, Jeanne Wilson-Rawls, Kenro Kusumi
المصدر: PLoS ONE, Vol 9, Iss 8, p e105004 (2014)
بيانات النشر: Public Library of Science (PLoS), 2014.
سنة النشر: 2014
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: Lizards, which are amniote vertebrates like humans, are able to lose and regenerate a functional tail. Understanding the molecular basis of this process would advance regenerative approaches in amniotes, including humans. We have carried out the first transcriptomic analysis of tail regeneration in a lizard, the green anole Anolis carolinensis, which revealed 326 differentially expressed genes activating multiple developmental and repair mechanisms. Specifically, genes involved in wound response, hormonal regulation, musculoskeletal development, and the Wnt and MAPK/FGF pathways were differentially expressed along the regenerating tail axis. Furthermore, we identified 2 microRNA precursor families, 22 unclassified non-coding RNAs, and 3 novel protein-coding genes significantly enriched in the regenerating tail. However, high levels of progenitor/stem cell markers were not observed in any region of the regenerating tail. Furthermore, we observed multiple tissue-type specific clusters of proliferating cells along the regenerating tail, not localized to the tail tip. These findings predict a different mechanism of regeneration in the lizard than the blastema model described in the salamander and the zebrafish, which are anamniote vertebrates. Thus, lizard tail regrowth involves the activation of conserved developmental and wound response pathways, which are potential targets for regenerative medical therapies.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1932-6203
Relation: http://europepmc.org/articles/PMC4139331?pdf=render; https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0105004
URL الوصول: https://doaj.org/article/268a2c826f8648f1a3e049ebcb83ce71
رقم الأكسشن: edsdoj.268a2c826f8648f1a3e049ebcb83ce71
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19326203
DOI:10.1371/journal.pone.0105004