دورية أكاديمية
Circ_0003645 serves as miR-335-5p sponge to promote the biological process of diffuse large B-cell lymphoma by upregulating NFIB
| العنوان: | Circ_0003645 serves as miR-335-5p sponge to promote the biological process of diffuse large B-cell lymphoma by upregulating NFIB |
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| المؤلفون: | Dayi Lin, Ya Wang, Linian Lei, Congmeng Lin |
| المصدر: | Autoimmunity, Vol 55, Iss 2, Pp 127-135 (2022) |
| بيانات النشر: | Taylor & Francis Group, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Internal medicine |
| مصطلحات موضوعية: | diffuse large b-cell lymphoma, circ_0003645, mir-335-5p, nfib, Internal medicine, RC31-1245 |
| الوصف: | Background Circular RNAs (circRNAs) are critical regulators for the development of many tumours, including diffuse large B-cell lymphoma (DLBCL). However, the role and mechanism of circ_0003645 in DLBCL progression remains obscure. Methods Quantitative real-time PCR was performed to measure the expression of circ_0003645, microRNA (miR)-335-5p and nuclear factor I/B (NFIB). Cell viability, apoptosis and cell cycle were measured by cell counting kit 8 assay and flow cytometry. Protein expression was assessed using western blot analysis, and cell glycolysis was evaluated by detecting glucose consumption and ATP/ADP ratios. Besides, dual-luciferase reporter assay and RIP assay were used to confirm RNA interaction. Results Our data showed that circ_0003645 expression was significantly upregulated in DLBCL tumour tissues. After circ_0003645 knockdown, the viability, cell cycle and glycolysis of DLBCL cells were inhibited, while cell apoptosis was promoted. MiR-335-5p could be sponged by circ_0003645, and NFIB was confirmed to be a downstream target of miR-335-5p. Function analysis revealed that anti-miR-335-5p reversed the regulation of si-circ_0003645 on DLBCL cell progression, and NFIB overexpression also abolished miR-335-5p-mediated the biological functions of DLBCL cells. Conclusion The present study revealed that circ_0003645 promoted the proliferation and glycolysis of DLBCL cells by the miR-335-5p/NFIB axis, which might provide a novel insight for DLBCL treatment. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0891-6934 1607-842X 08916934 |
| Relation: | https://doaj.org/toc/0891-6934; https://doaj.org/toc/1607-842X |
| DOI: | 10.1080/08916934.2021.2023863 |
| URL الوصول: | https://doaj.org/article/2725611d7a624dd08aa173c5bb7fd137 |
| رقم الأكسشن: | edsdoj.2725611d7a624dd08aa173c5bb7fd137 |
| قاعدة البيانات: | Directory of Open Access Journals |
PDF full text from Publisher | تدمد: | 08916934 1607842X |
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| DOI: | 10.1080/08916934.2021.2023863 |