دورية أكاديمية
The immobilization of fibronectin- and fibroblast growth factor 2-derived peptides on a culture plate supports the attachment and proliferation of human pluripotent stem cells
العنوان: | The immobilization of fibronectin- and fibroblast growth factor 2-derived peptides on a culture plate supports the attachment and proliferation of human pluripotent stem cells |
---|---|
المؤلفون: | Ahmed Abdal Dayem, Jihye Won, Hui-Gwan Goo, Gwang-Mo Yang, Dong Sik Seo, Byeong-Min Jeon, Hye Yeon Choi, Sang Eun Park, Kyung Min Lim, Seon-Ho Jang, Soo Bin Lee, Sang Baek Choi, Kyeongseok Kim, Geun-Ho Kang, Gyu-Bum Yeon, Dae-Sung Kim, Ssang-Goo Cho |
المصدر: | Stem Cell Research, Vol 43, Iss , Pp - (2020) |
بيانات النشر: | Elsevier, 2020. |
سنة النشر: | 2020 |
المجموعة: | LCC:Biology (General) |
مصطلحات موضوعية: | Biology (General), QH301-705.5 |
الوصف: | Pluripotent stem cells (PSCs) offer a promising tool for regenerative medicine. The clinical application of PSCs inevitably requires a large-scale culture in a highly defined environment. The present study aimed to devise defined coating materials for the efficient adhesion and proliferation of human PSCs (hPSCs). We tested the activity of seven fibronectin-derived peptides and three laminin-derived peptides for the attachment and proliferation of hPSCs through their immobilization on the bottom of culture dishes by creating a fusion protein with the mussel adhesion protein. Among the extracellular matrix (ECM) mimetics tested, one fibronectin-derived peptide, PHSRN-GRGDSP, significantly promoted adhesion, enhanced alkaline phosphatase activity, and increased pluripotency-related gene expression in hPSCs compared to Matrigel. Furthermore, co-immobilization of a particular canofin peptide derived from fibroblast growth factor 2 increased pluripotency marker expression, which may offer the possibility of culture without growth factor supplementation. Our findings afford a novel defined condition for the efficient culture of hPSCs and may be utilized in future clinical applications. Keywords: Human induced pluripotent stem cell, Stem cell, Niche, ECM motif, Proliferation, Pluripotency, Adhesion |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1873-5061 |
Relation: | http://www.sciencedirect.com/science/article/pii/S1873506120300027; https://doaj.org/toc/1873-5061 |
DOI: | 10.1016/j.scr.2020.101700 |
URL الوصول: | https://doaj.org/article/2765f193a58247c0b7741b8ca3526f65 |
رقم الأكسشن: | edsdoj.2765f193a58247c0b7741b8ca3526f65 |
قاعدة البيانات: | Directory of Open Access Journals |
كن أول من يترك تعليقا!