دورية أكاديمية
Innate Immune Response of TmToll-3 Following Systemic Microbial Infection in Tenebrio molitor
| العنوان: | Innate Immune Response of TmToll-3 Following Systemic Microbial Infection in Tenebrio molitor |
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| المؤلفون: | Maryam Ali Mohammadie Kojour, Ho Am Jang, Yong Seok Lee, Yong Hun Jo, Yeon Soo Han |
| المصدر: | International Journal of Molecular Sciences, Vol 24, Iss 7, p 6751 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | Toll-3, Tenebrio molitor, microbial infection, RNAi, antimicrobial peptides, AMP assay, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Although Toll-like receptors have been widely identified and functionally characterized in mammalian models and Drosophila, the immunological function of these receptors in other insects remains unclear. Here, we explored the relevant innate immune response of Tenebrio molitor (T. molitor) Toll-3 against Gram-negative bacteria, Gram-positive bacteria, and fungal infections. Our findings indicated that TmToll-3 expression was mainly induced by Candida albicans infections in the fat bodies, gut, Malpighian tubules, and hemolymph of young T. molitor larvae. Surprisingly, Escherichia coli systemic infection caused mortality after TmToll-3 knockdown via RNA interference (RNAi) injection, which was not observed in the control group. Further analyses indicated that in the absence of TmToll-3, the final effector of the Toll signaling pathway, antimicrobial peptide (AMP) genes and relevant transcription factors were significantly downregulated after E. coli challenge. Our results indicated that the expression of almost all AMP genes was suppressed in silenced individuals, whereas the expression of relevant genes was positively regulated after fungal injection. Therefore, this study revealed the immunological involvement of TmToll-3 in T. molitor in response to systematic infections. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/24/7/6751; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms24076751 |
| URL الوصول: | https://doaj.org/article/d279216ae3a043eab9ab5b7cb5c7aeee |
| رقم الأكسشن: | edsdoj.279216ae3a043eab9ab5b7cb5c7aeee |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 16616596 |
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| DOI: | 10.3390/ijms24076751 |