دورية أكاديمية
Real-time detection of Seneca Valley virus by one-tube RPA-CRISPR/Cas12a assay
| العنوان: | Real-time detection of Seneca Valley virus by one-tube RPA-CRISPR/Cas12a assay |
|---|---|
| المؤلفون: | Lei Ma, Mengjie Zhu, Qingfeng Meng, Yao Wang, Xueping Wang |
| المصدر: | Frontiers in Cellular and Infection Microbiology, Vol 13 (2024) |
| بيانات النشر: | Frontiers Media S.A., 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Microbiology |
| مصطلحات موضوعية: | Senecavirus A, recombinase polymerase amplification, CRISPR/Cas12a, detection, swine, Microbiology, QR1-502 |
| الوصف: | IntroductionSenecavirus A (SVA) is a highly contagious virus that causes vesicular disease in pigs. At present, laboratory detection methods, such as virus isolation and polymerase chain reaction (PCR), required precision instruments and qualified personnel, making them unsuitable for point-of-care tests (POCT). Fortunately, the emergence of CRISPR/Cas system has provided new opportunities for fast and efficient pathogen detection.MethodsThis study successfully developed a precise and sensitive detection platform for diagnosing SVA by combining the CRISPR system with recombinase polymerase amplification (RPA). ResultsThe minimum detection limit of the assay was 10 copies of the SVA genome. Meanwhile, the assay demonstrated high specificity. To validate the effectiveness of this system, we tested 85 swine clinical samples and found that the fluorescence method had a 100% coincidence rate compared to RT-qPCR. DiscussionOverall, the RPA-CRISPR/Cas12a assay established in our study is a highly effective method for detecting SVA and holds great potential for practical applications in the resource-limited settings. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2235-2988 46656189 |
| Relation: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1305222/full; https://doaj.org/toc/2235-2988 |
| DOI: | 10.3389/fcimb.2023.1305222 |
| URL الوصول: | https://doaj.org/article/288e66aae6c24cee81b46656189a2cff |
| رقم الأكسشن: | edsdoj.288e66aae6c24cee81b46656189a2cff |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22352988 46656189 |
|---|---|
| DOI: | 10.3389/fcimb.2023.1305222 |