دورية أكاديمية
Absorption and Excretion of Glucosinolates and Isothiocyanates after Ingestion of Broccoli (Brassica oleracea L. var italica) Leaf Flour in Mice: A Preliminary Study
| العنوان: | Absorption and Excretion of Glucosinolates and Isothiocyanates after Ingestion of Broccoli (Brassica oleracea L. var italica) Leaf Flour in Mice: A Preliminary Study |
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| المؤلفون: | Tânia Martins, Tiago Ferreira, Bruno Colaço, Beatriz Medeiros-Fonseca, Maria de Lurdes Pinto, Ana Novo Barros, Carlos Venâncio, Eduardo Rosa, Luís Miguel Antunes, Paula Alexandra Oliveira, Maria João Pires |
| المصدر: | Nutraceuticals, Vol 3, Iss 4, Pp 540-555 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Nutrition. Foods and food supply LCC:Nutritional diseases. Deficiency diseases |
| مصطلحات موضوعية: | broccoli by-products, bioavailability, health, C57BL/6J mice, Nutrition. Foods and food supply, TX341-641, Nutritional diseases. Deficiency diseases, RC620-627 |
| الوصف: | During the harvesting of the broccoli plant, the leaves are discarded, being considered a by-product that may be up to 47% of total broccoli biomass, representing a large amount of wasted material. The use of broccoli leaves is of great interest in the sense that this wasted material is rich in health promoter compounds, such as isothiocyanates. In this study, C57BL/6J mice ingested 790 mg/kg broccoli leaf flour, and the presence of glucosinolates and isothiocyanates in the plasma, liver, kidney, adipose tissue, faeces and urine was analysed at 1, 2, 4, 8, 12 and 24 h post-ingestion. In plasma, only glucoerucin (GE), glucobrassicin (GB), sulforaphane (SFN) and indol-3-carbinol (I3C) were detected, and all four compounds peaked between 4 and 8 h after ingestion. The compounds SFN, SFN-glutathione (SFN-GSH), SFN–cysteine (SFN-CYS) and SFN-N-acetyl-cysteine (SFN-NAC) were excreted in faeces at high levels, while glucoraphanin (GR), the precursor of SFN, was not detected in any biological samples other than urine. In the liver, the compounds GE, SFN-CYS, SFN-NAC and I3C were detected, while in the kidney, only GE, GB and SFN-GSH were present. None of the glucosinolates and isothiocyanates analysed were detected in fat tissue. These results demonstrate that glucosinolates and their derivatives were absorbed into the bloodstream and were bioavailable after ingestion of powdered broccoli leaves. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1661-3821 |
| Relation: | https://www.mdpi.com/1661-3821/3/4/39; https://doaj.org/toc/1661-3821 |
| DOI: | 10.3390/nutraceuticals3040039 |
| URL الوصول: | https://doaj.org/article/2aaf42cbd35e4c62885d9562a584b582 |
| رقم الأكسشن: | edsdoj.2aaf42cbd35e4c62885d9562a584b582 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 16613821 |
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| DOI: | 10.3390/nutraceuticals3040039 |