دورية أكاديمية
5-BDBD ameliorates an OVA-induced allergic asthma by the reduction of Th2 cytokines production
| العنوان: | 5-BDBD ameliorates an OVA-induced allergic asthma by the reduction of Th2 cytokines production |
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| المؤلفون: | Bing Hu, Xiaoqian Feng, Li Wang, Yinli Song, Xiuqin Ni |
| المصدر: | Iranian Journal of Basic Medical Sciences, Vol 21, Iss 4, Pp 364-369 (2018) |
| بيانات النشر: | Mashhad University of Medical Sciences, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Medicine |
| مصطلحات موضوعية: | 5-BDBD, Asthma, Gata-3, T-bet, Th2 cells, Medicine |
| الوصف: | Objective(s): P2X4R is expressed in immunocyte and lung tissues. It has been a focus in inflammatory responses recently. This study investigated whether blockage of P2X4R attenuates allergic inflammation by modulating T cell response in ovalbumin-sensitized mice. Materials and Methods: Ovalbumin was used to sensitize and challenge for a mouse model. Intranasal application of 5-BDBD, P2X4R antagonist, were performed 3 hr before each airway allergen challenge. The lung was evaluated for P2X4R by real-time PCR and immunofluorescence. Th1/Th2 cytokines in bronchoalveolar lavage fluid were measured by ELISA. T-bet, Gata-3, and p-p38 MAPK were measured by Western blot or real-time PCR. Results: P2X4R was overexpressed in the lung after allergen challenge compared with the control group. Blockage of P2X4R decreased inflammation in the lung, IL-4 expression was reduced as well as IL-5; IFN-γ expression was elevated in BALF in ovalbumin-sensitized mice. Moreover, blockage of P2X4R inhibited ovalbumin-induced increased Gata-3 level and decreased T-bet level. Conclusion: These findings suggest that 5-BDBD ameliorates an ovalbumin-induced asthmatic attack by the downregulation of cytokines related to the Th2 cell. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2008-3866 2008-3874 |
| Relation: | http://ijbms.mums.ac.ir/article_10373_238968e1f33d40510d4441cf2cb53126.pdf; https://doaj.org/toc/2008-3866; https://doaj.org/toc/2008-3874 |
| DOI: | 10.22038/ijbms.2018.25731.6345 |
| URL الوصول: | https://doaj.org/article/eec2e33717c84630872604362d801c5f |
| رقم الأكسشن: | edsdoj.2e33717c84630872604362d801c5f |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20083866 20083874 |
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| DOI: | 10.22038/ijbms.2018.25731.6345 |