دورية أكاديمية
Progressive Motor and Non-Motor Symptoms in Park7 Knockout Zebrafish
| العنوان: | Progressive Motor and Non-Motor Symptoms in Park7 Knockout Zebrafish |
|---|---|
| المؤلفون: | Lakshmi Narasimha Murthy Chavali, Ingeborg Yddal, Ersilia Bifulco, Simen Mannsåker, Dagne Røise, Jack O. Law, Ann-Kristin Frøyset, Sushma N. Grellscheid, Kari E. Fladmark |
| المصدر: | International Journal of Molecular Sciences, Vol 24, Iss 7, p 6456 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | Park7, DJ-1, zebrafish, Parkinson’s disease, behavior, NAD metabolism, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | DJ-1 is a redox sensitive protein with a wide range of functions related to oxidative stress protection. Mutations in the park7 gene, which codes for DJ-1 are associated with early onset familial Parkinson’s disease and increased astrocytic DJ-1 levels are found in pathologic tissues from idiopathic Parkinson’s disease. We have previously established a DJ-1 knockout zebrafish line that developed normally, but with aging the DJ-1 null fish had a lowered level of tyrosine hydroxylase, respiratory mitochondrial failure and a lower body mass. Here we have examined the DJ-1 knockout from the early adult stage and show that loss of DJ-1 results in a progressive, age-dependent increase in both motoric and non-motoric symptoms associated to Parkinson’s disease. These changes coincide with changes in mitochondrial and mitochondrial associated proteins. Recent studies have suggested that a decline in NAD+ can contribute to Parkinson’s disease and that supplementation of NAD+ precursors may delay disease progression. We found that the brain NAD+/NADH ratio decreased in aging zebrafish but did not correlate with DJ-1 induced altered behavior. Differences were first observed at the late adult stage in which NAD+ and NADPH levels were decreased in DJ-1 knockouts. Considering the experimental power of zebrafish and the development of Parkinson’s disease-related symptoms in the DJ-1 null fish, this model can serve as a useful tool both to understand the progression of the disease and the effect of suggested treatments. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/24/7/6456; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms24076456 |
| URL الوصول: | https://doaj.org/article/2f6af4fcf06841378c893b816c97c16c |
| رقم الأكسشن: | edsdoj.2f6af4fcf06841378c893b816c97c16c |
| قاعدة البيانات: | Directory of Open Access Journals |
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