دورية أكاديمية
Adipose Tissue-Derived Mesenchymal Cells Support Skin Reepithelialization through Secretion of KGF-1 and PDGF-BB: Comparison with Dermal Fibroblasts
| العنوان: | Adipose Tissue-Derived Mesenchymal Cells Support Skin Reepithelialization through Secretion of KGF-1 and PDGF-BB: Comparison with Dermal Fibroblasts |
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| المؤلفون: | Vassilia-Ismini Alexaki, Despoina Simantiraki, Marianna Panayiotopoulou, Olga Rasouli, Maria Venihaki, Ourania Castana, Dimitrios Alexakis, Marilena Kampa, Efstathios N. Stathopoulos, Elias Castanas |
| المصدر: | Cell Transplantation, Vol 21 (2012) |
| بيانات النشر: | SAGE Publishing, 2012. |
| سنة النشر: | 2012 |
| المجموعة: | LCC:Medicine |
| مصطلحات موضوعية: | Medicine |
| الوصف: | Epidermal organization and homeostasis are regulated by mesenchymal influences through paracrine actions. Until today, dermal fibroblasts (DFs) are used in the “dermal” layer to support keratinocyte growth in vitro in dermal and skin substitutes. In the present work, we used human adipose tissue-derived mesenchymal cells (ADMCs) as a support of keratinocyte growth in vitro (in monolayer culture and in 3D skin cell culture models) and in vivo (mouse wound healing models) and compared our findings with those obtained using dermal fibroblasts. ADMCs induce reepithelialization during wound healing more efficiently than DFs, by enhancing keratinocyte proliferation through cell cycle progression, and migration. This effect is mediated (at least partially) by a paracrine action of KGF-1 and PDGF-BB, which are more prominently expressed in ADMCs than in DFs. Furthermore, replacement of DFs by ADMCs in the dermal compartment of organotypic skin cultures leads to an artificial epidermis resembling to that of normal skin, concerning the general histology, although with a higher expression of cytokeratins 5 and 19. In Rag1 knockout mice, ADMCs induced a more rapid reepithelialization and a more effective wound healing, compared to dermal fibroblasts. In conclusion, we provide evidence that ADMCs can serve as supportive cells for primary keratinocyte cultures. In addition, because of their abundance and the great cell yield achieved during ADMC isolation, they represent an interesting cell source, with potential aspects for clinical use. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0963-6897 1555-3892 |
| Relation: | https://doaj.org/toc/0963-6897; https://doaj.org/toc/1555-3892 |
| DOI: | 10.3727/096368912X637064 |
| URL الوصول: | https://doaj.org/article/e3018597abfb4f75afe7e2929e63234a |
| رقم الأكسشن: | edsdoj.3018597abfb4f75afe7e2929e63234a |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 09636897 15553892 |
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| DOI: | 10.3727/096368912X637064 |