دورية أكاديمية
The effect of postprandial in vivo and experimental in vitro hyperlipidemia on human peripheral blood monocytes
العنوان: | The effect of postprandial in vivo and experimental in vitro hyperlipidemia on human peripheral blood monocytes |
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المؤلفون: | Manescu Ion Bogdan, Manescu Mariuca, Preda Elena Cristina, Manu Doina Ramona, Dobreanu Minodora |
المصدر: | Acta Marisiensis - Seria Medica, Vol 68, Iss 4, Pp 172-178 (2022) |
بيانات النشر: | Sciendo, 2022. |
سنة النشر: | 2022 |
المجموعة: | LCC:Medicine |
مصطلحات موضوعية: | cd14 cd11b monocyte, lipids, cell activation, postprandial hyperlipidemia, bodipy, Medicine |
الوصف: | Objective: In this study, we aimed to investigate the effect of transient postprandial in vivo and prolonged experimental in vitro hyperlipidemia on human peripheral blood monocytes. Methods: Peripheral blood was collected from seven healthy subjects after an overnight fast and three hours after a standardized high-fat meal. Both fasting and postprandial samples were stained for surface markers CD14/CD11b and intracellular lipids using BODIPY493/503. Postprandial samples only were used for isolation of peripheral blood mononuclear cells that were further incubated overnight with postprandial hyperlipidemic autologous plasma, then stained as described above. All samples were analyzed on a FACSAria III flow cytometer. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 2668-7763 |
Relation: | https://doaj.org/toc/2668-7763 |
DOI: | 10.2478/amma-2022-0026 |
URL الوصول: | https://doaj.org/article/33ba8e8794594232b13e0718a3889387 |
رقم الأكسشن: | edsdoj.33ba8e8794594232b13e0718a3889387 |
قاعدة البيانات: | Directory of Open Access Journals |
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