دورية أكاديمية
The adjuvant effect of polymuramil, a NOD1 and NOD2 agonist, differs when immunizing mice of different inbred lines with nonstructural hepatitis C virus (Flaviviridae: Hepacivirus)proteins and is synergistically enhanced in combination with pyrogenalum, a TLR4 agonist
| العنوان: | The adjuvant effect of polymuramil, a NOD1 and NOD2 agonist, differs when immunizing mice of different inbred lines with nonstructural hepatitis C virus (Flaviviridae: Hepacivirus)proteins and is synergistically enhanced in combination with pyrogenalum, a TLR4 agonist |
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| المؤلفون: | Ekaterina I. Lesnova, Olga V. Masalova, Kristina Yu. Permyakova, Natalia A. Demidova, Vladimir T. Valuev-Elliston, Alexandr V. Ivanov, Alla A. Kushch |
| المصدر: | Вопросы вирусологии, Vol 68, Iss 4, Pp 315-326 (2023) |
| بيانات النشر: | Central Research Institute for Epidemiology, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Microbiology |
| مصطلحات موضوعية: | hepatitis c, hepatitis c virus, non-structural hepatitis c virus proteins, balb/c, dba/2j and c57bl/6 mice, humoral and cellular immune responses, polymuramyl, pyrogenalum, synergistic adjuvant effect, Microbiology, QR1-502 |
| الوصف: | Introduction. Hepatitis C is a liver disease with high chronicity, the cause of cirrhosis and hepatocarcinoma. The main obstacle to controlling hepatitis C is the lack of vaccines. The aim of the work was to compare the immunogenic activity of nonstructural recombinant proteins NS3, NS4 and NS5B of hepatitis C virus (HCV) as components of a subunit candidate vaccine and to analyze the adjuvant properties of two available commercial drugs, polymuramil and pyrogenalum. Materials and methods. BALB/c, DBA/2J and C57BL/6 mice were immunized with nonstructural proteins without adjuvants or with polymuramyl (NOD1 and NOD2 agonist) and pyrogenalum (TLR-4 agonist). The activity of antibodies was determined in ELISA, the cellular response by antigen-specific lymphocyte proliferation and by production of IFN- in vitro. Results. Recombinant proteins showed different immunogenicity. NS4 induced antibodies more efficiently than NS3 and NS5B. Significant differences were found in the immune response of three inbred lines mice: the level of IFN- in BALB/c and DBA/2J mice induced by NS5B protein was 30 times higher than in C57Bl/6 mice. In contrast, the induction of antibodies in BALB/c mice was lower than in C57Bl/6 and DBA/2J. Polymuramil did not increase the humoral response to NS5B and enhanced the cellular response only in C57BL/6 mice. The combined use of polymuramil with pyrogenalum significantly increased both the humoral and cellular response of mice to all recombinant HCV proteins. Conclusion. Different immunogenic properties and different functions of recombinant non-structural HCV proteins indicate the feasibility of their combined inclusion in subunit vaccines. It was established for the first time that immunization with HCV proteins with a complex adjuvant (polymuramyl + pyrogenalum) has a synergistic effect, significantly exceeding the effect of each of them separately. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Russian |
| تدمد: | 0507-4088 2411-2097 |
| Relation: | https://virusjour.crie.ru/jour/article/viewFile/13159/799; https://doaj.org/toc/0507-4088; https://doaj.org/toc/2411-2097 |
| DOI: | 10.36233/0507-4088-183 |
| URL الوصول: | https://doaj.org/article/33c47a539ad64c0596a2cead6db24048 |
| رقم الأكسشن: | edsdoj.33c47a539ad64c0596a2cead6db24048 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 05074088 24112097 |
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| DOI: | 10.36233/0507-4088-183 |