دورية أكاديمية
Combining Solid and Liquid Biopsy for Therapy Monitoring in Esophageal Cancer
| العنوان: | Combining Solid and Liquid Biopsy for Therapy Monitoring in Esophageal Cancer |
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| المؤلفون: | Florian Richter, Clara Henssen, Tim Alexander Steiert, Tobias Meissner, Anne-Sophie Mehdorn, Christoph Röcken, Andre Franke, Jan-Hendrik Egberts, Thomas Becker, Susanne Sebens, Michael Forster |
| المصدر: | International Journal of Molecular Sciences, Vol 24, Iss 13, p 10673 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | EC, cell-free DNA, cfDNA, circulating tumor DNA, ctDNA, NGS, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Esophageal cancer (EC) has one of the highest mortality rates among cancers, making it imperative that therapies are optimized and dynamically adapted to individuals. In this regard, liquid biopsy is an increasingly important method for residual disease monitoring. However, conflicting detection rates (14% versus 60%) and varying cell-free circulating tumor DNA (ctDNA) levels (0.07% versus 0.5%) have been observed in previous studies. Here, we aim to resolve this discrepancy. For 19 EC patients, a complete set of cell-free DNA (cfDNA), formalin-fixed paraffin-embedded tumor tissue (TT) DNA and leukocyte DNA was sequenced (139 libraries). cfDNA was examined in biological duplicates and/or longitudinally, and TT DNA was examined in technical duplicates. In baseline cfDNA, mutations were detected in 12 out of 19 patients (63%); the median ctDNA level was 0.4%. Longitudinal ctDNA changes were consistent with clinical presentation. Considerable mutational diversity was observed in TT, with fewer mutations in cfDNA. The most recurrently mutated genes in TT were TP53, SMAD4, TSHZ3, and SETBP1, with SETBP1 being reported for the first time. ctDNA in blood can be used for therapy monitoring of EC patients. However, a combination of solid and liquid samples should be used to help guide individualized EC therapy. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 24131067 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/24/13/10673; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms241310673 |
| URL الوصول: | https://doaj.org/article/38241694d50b4b818ec07b8bb30ed137 |
| رقم الأكسشن: | edsdoj.38241694d50b4b818ec07b8bb30ed137 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 24131067 14220067 16616596 |
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| DOI: | 10.3390/ijms241310673 |