دورية أكاديمية
Use of High-Refractive Index Hydrogels and Tissue Clearing for Large Biological Sample Imaging
| العنوان: | Use of High-Refractive Index Hydrogels and Tissue Clearing for Large Biological Sample Imaging |
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| المؤلفون: | Alexander Richardson, Sandra Fok, Victoria Lee, Kerry-Anne Rye, Nick Di Girolamo, Blake J. Cochran |
| المصدر: | Gels, Vol 8, Iss 1, p 32 (2022) |
| بيانات النشر: | MDPI AG, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Science LCC:Chemistry LCC:Inorganic chemistry LCC:General. Including alchemy |
| مصطلحات موضوعية: | hydrogel, tissue clearing, sample preparation, light sheet microscopy, Science, Chemistry, QD1-999, Inorganic chemistry, QD146-197, General. Including alchemy, QD1-65 |
| الوصف: | Recent advances in tissue clearing and light sheet fluorescence microscopy have improved insights into and understanding of tissue morphology and disease pathology by imaging large samples without the requirement of histological sectioning. However, sample handling and conservation of sample integrity during lengthy staining and acquisition protocols remains a challenge. This study overcomes these challenges with acrylamide hydrogels synthesised to match the refractive index of solutions typically utilised in aqueous tissue clearing protocols. These hydrogels have a high-water content (82.0 ± 3.7% by weight). The gels are stable over time and FITC-IgG readily permeated into and effluxed out of them. Whilst the gels deformed and/or swelled over time in some commonly used solutions, this was overcome by using a previously described custom refractive index matched solution. To validate their use, CUBIC cleared mouse tissues and whole embryos were embedded in hydrogels, stained using fluorescent small molecule dyes, labels and antibodies and successfully imaged using light sheet fluorescence microscopy. In conclusion, the high water content, high refractive index hydrogels described in this study have broad applicability to research that delves into pathophysiological processes by stabilising and protecting large and fragile samples. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2310-2861 |
| Relation: | https://www.mdpi.com/2310-2861/8/1/32; https://doaj.org/toc/2310-2861 |
| DOI: | 10.3390/gels8010032 |
| URL الوصول: | https://doaj.org/article/3c4ee6692ecb49b7a718f1e1eaf0226c |
| رقم الأكسشن: | edsdoj.3c4ee6692ecb49b7a718f1e1eaf0226c |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 23102861 |
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| DOI: | 10.3390/gels8010032 |