دورية أكاديمية

Evaluation of Yersinia Enterocolitica Diagnostic Approaches

التفاصيل البيبلوغرافية
العنوان: Evaluation of Yersinia Enterocolitica Diagnostic Approaches
المؤلفون: R Bakhtiary, A Brahmeh, S Jadidy, S Eshraghi, F Matin, MR Khorramizadeh, MM Soltan Dallal, F Saberpoor, SZ Rouhani Rankouhi
المصدر: پیاورد سلامت, Vol 1, Iss 2, Pp 5-10 (2008)
بيانات النشر: Tehran University of Medical Sciences, 2008.
سنة النشر: 2008
المجموعة: LCC:Public aspects of medicine
مصطلحات موضوعية: Yersinia Enterocolitica, Pathogenesis, PCR, Public aspects of medicine, RA1-1270
الوصف: Background and Aim: Yersinia enterocolitica is a Gram-negative bacterium which its strains are involved in human diseases. To differentiate among pathogenic and non-pathogenic types, tests such as Congo Red absorption, Crystal Violet, and Calcium Dependency test are used. These tests are based on existence of 70-75 kb plasmids and sometimes, with respect to plasmids instability, we will face false negative results. Therefore, by setting up a methodology based on stable chromosomal genes of pathogenic agent we can overcome this hurdle. The goal of this survey was comparison among routine and molecular diagnostic approaches in the identification of Y. enterocolitica pathogenic strains.Materials and Methods: Some Gram-negative bacteria from family Enterobacteriacea and some Y. enterocolitica strains isolated of human beings and environment were evaluated.Results: Obtained results showed that 4 Y. enterocolitica strains isolated of human beings were PCR positive while PCR results of environmental strains, one human strain and non-Yersinia strains were negative.Conclusion: The mentioned approach can be used as a method to differentiate among pathogenic and non-pathogenic strains of Y. enterocolitica.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: Persian
تدمد: 1735-8132
2008-2665
Relation: http://journals.tums.ac.ir/PdfMed.aspx?pdf_med=/upload_files/pdf/12225.pdf&manuscript_id=12225; https://doaj.org/toc/1735-8132; https://doaj.org/toc/2008-2665
URL الوصول: https://doaj.org/article/3e7aca303626456c892646811a835926
رقم الأكسشن: edsdoj.3e7aca303626456c892646811a835926
قاعدة البيانات: Directory of Open Access Journals