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Genome duplication in Leishmania major relies on persistent subtelomeric DNA replication

التفاصيل البيبلوغرافية
العنوان: Genome duplication in Leishmania major relies on persistent subtelomeric DNA replication
المؤلفون: Jeziel Dener Damasceno, Catarina A Marques, Dario Beraldi, Kathryn Crouch, Craig Lapsley, Ricardo Obonaga, Luiz RO Tosi, Richard McCulloch
المصدر: eLife, Vol 9 (2020)
بيانات النشر: eLife Sciences Publications Ltd, 2020.
سنة النشر: 2020
المجموعة: LCC:Medicine
LCC:Science
LCC:Biology (General)
مصطلحات موضوعية: Leishmania, DNA replication, origin, subtelomere, Medicine, Science, Biology (General), QH301-705.5
الوصف: DNA replication is needed to duplicate a cell’s genome in S phase and segregate it during cell division. Previous work in Leishmania detected DNA replication initiation at just a single region in each chromosome, an organisation predicted to be insufficient for complete genome duplication within S phase. Here, we show that acetylated histone H3 (AcH3), base J and a kinetochore factor co-localise in each chromosome at only a single locus, which corresponds with previously mapped DNA replication initiation regions and is demarcated by localised G/T skew and G4 patterns. In addition, we describe previously undetected subtelomeric DNA replication in G2/M and G1-phase-enriched cells. Finally, we show that subtelomeric DNA replication, unlike chromosome-internal DNA replication, is sensitive to hydroxyurea and dependent on 9-1-1 activity. These findings indicate that Leishmania’s genome duplication programme employs subtelomeric DNA replication initiation, possibly extending beyond S phase, to support predominantly chromosome-internal DNA replication initiation within S phase.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2050-084X
Relation: https://elifesciences.org/articles/58030; https://doaj.org/toc/2050-084X
DOI: 10.7554/eLife.58030
URL الوصول: https://doaj.org/article/4001a244fbe04e1ca359036aba6df1ad
رقم الأكسشن: edsdoj.4001a244fbe04e1ca359036aba6df1ad
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:2050084X
DOI:10.7554/eLife.58030