دورية أكاديمية
Plasmodium Berghei ANKA Infection in ICR Mice as a Model of Cerebral Malaria
| العنوان: | Plasmodium Berghei ANKA Infection in ICR Mice as a Model of Cerebral Malaria |
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| المؤلفون: | F Othman, TH Tie, M Jabbarzare, MF Yam, H Talib, WC Chong, K Hasballah, SS Fazalul Rahiman, R Basir, MAM Moklas, WO Abdullah, Z Ahmad |
| المصدر: | Iranian Journal of Parasitology, Vol 7, Iss 4, Pp 62-74 (2012) |
| بيانات النشر: | Tehran University of Medical Sciences, 2012. |
| سنة النشر: | 2012 |
| المجموعة: | LCC:Infectious and parasitic diseases |
| مصطلحات موضوعية: | Animal Model, Malaria, Plasmodium Berghei, ICR Mice, Infectious and parasitic diseases, RC109-216 |
| الوصف: | Background: Animal models with various combination of host-parasite have long been employed to study malaria pathogenesis. Here, we describe the combination of Plasmodium berghei ANKA infection in inbred ICR mice as a model of cerebral malaria (CM).Methods: Infection in mice was initiated by intraperitoneal injection of 2 x 107 (0.2ml) parasitized red blood cells (PRBCs).Results: This model can produce a severe degree of infection presented by the high degree of parasitaemia followed by death 6-7 days post infection. Severe anemia, splenomegaly, hepatomegaly and discolourations of major organs were observed. Histopathological findings revealed several important features mimicking human CM including, microvascular sequestration of PRBCs in major organs, particularly in the brain, hypertrophy and hyperplasia of the kupffer cells in the liver, pulmonary edema and hyaline membrane formation in the lungs and haemorrhages in the kidney’s medulla and cortex. Proinflammatory cytokines TNFα, IFNγ, IL-1, IL-6 and IL-18, and anti-inflammatory cytokine IL-10 were all found to be elevated in the plasma of infected mice.Conclusion: This model can reproduce many of the important features of CM and therefore can be used as a tool to advance our understanding of the disease pathogenesis. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1735-7020 2008-238X |
| Relation: | http://ijpa.tums.ac.ir/index.php/ijpa/article/view/470/344; https://doaj.org/toc/1735-7020; https://doaj.org/toc/2008-238X |
| URL الوصول: | https://doaj.org/article/42a3800234e14f918139712cce91c2eb |
| رقم الأكسشن: | edsdoj.42a3800234e14f918139712cce91c2eb |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 17357020 2008238X |
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