دورية أكاديمية
Rapid detection of colistin resistance protein MCR-1 by LC–MS/MS
| العنوان: | Rapid detection of colistin resistance protein MCR-1 by LC–MS/MS |
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| المؤلفون: | Honghui Wang, Yong Chen, Jeffrey R. Strich, Steven K. Drake, Jung-Ho Youn, Avi Z. Rosenberg, Marjan Gucek, Patrick T. McGann, Anthony F. Suffredini, John P. Dekker |
| المصدر: | Clinical Proteomics, Vol 16, Iss 1, Pp 1-10 (2019) |
| بيانات النشر: | BMC, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Medicine |
| مصطلحات موضوعية: | Medicine |
| الوصف: | Abstract Background Colistin (polymyxin E) and polymixin B are important bactericidal antibiotics used in the treatment of serious infections caused by multi-drug resistant Gram-negative organisms. Transferrable plasmid-mediated colistin resistance, conferred by the product of the mcr-1 gene, has emerged as a global healthcare threat. Consequently, the rapid detection of the MCR-1 protein in clinical bacterial isolates has become increasingly important. We used a genoproteomic approach to identify unique peptides of the MCR-1 protein that could be detected rapidly by liquid chromatography tandem mass spectrometry (LC–MS/MS). Methods MCR-1 tryptic peptides that were efficiently ionized and readily detectable were characterized in a set of mcr-1-containing isolates with triple quadrupole LC–MS. Three optimal peptides were selected for the development of a rapid multiple reaction monitoring LC–MS/MS assay for the MCR-1 protein. To investigate the feasibility of rapid detection of the MCR-1 protein in bacterial isolates using this assay, a blinded 99-sample test set was built that included three additional mcr-1-containing clinical isolates tested in triplicate (9 samples) and 90 negative control isolates. Results All of the mcr-1-containing isolates in the test set were accurately identified with no false positive detections by three independent, blinded operators, yielding an overall performance of 100% sensitivity and specificity for multiple operators. Among the three peptides tested in this study, the best performing was DTFPQLAK. The isolate-to-result time for the assay as implemented is less than 90 min. Conclusions This work demonstrates the feasibility of rapid detection of the MCR-1 protein in bacterial isolates by LC–MS/MS. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1542-6416 1559-0275 |
| Relation: | http://link.springer.com/article/10.1186/s12014-019-9228-2; https://doaj.org/toc/1542-6416; https://doaj.org/toc/1559-0275 |
| DOI: | 10.1186/s12014-019-9228-2 |
| URL الوصول: | https://doaj.org/article/4331f9a41a214da3b0c2055a8a84357e |
| رقم الأكسشن: | edsdoj.4331f9a41a214da3b0c2055a8a84357e |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 15426416 15590275 |
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| DOI: | 10.1186/s12014-019-9228-2 |