دورية أكاديمية
Modified CLEC3A-Derived Antimicrobial Peptides Lead to Enhanced Antimicrobial Activity against Drug-Resistant Bacteria
| العنوان: | Modified CLEC3A-Derived Antimicrobial Peptides Lead to Enhanced Antimicrobial Activity against Drug-Resistant Bacteria |
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| المؤلفون: | Denise Meinberger, Marco G. Drexelius, Joshua Grabeck, Gabriele Hermes, Annika Roth, Dzemal Elezagic, Ines Neundorf, Thomas Streichert, Andreas R. Klatt |
| المصدر: | Antibiotics, Vol 12, Iss 10, p 1532 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Therapeutics. Pharmacology |
| مصطلحات موضوعية: | CLEC3A, C-type lectin, antimicrobial peptides, peptide modification, drug-resistant bacteria, MRSA, Therapeutics. Pharmacology, RM1-950 |
| الوصف: | Antimicrobial peptides (AMPs) represent a promising alternative to conventional antibiotics. Sequence changes can significantly improve the therapeutic properties of antimicrobial peptides. In our study, we apply different sequence modifications to enhance the performance of the CLEC3A-derived AMPs HT-16 and HT-47. We truncated their sequences, inserting a triple-glycine linker, adding an N-terminal tryptophan residue, and generating a D-amino acid variant, resulting in the generation of seven new peptides. We investigated their antimicrobial activity against gram-positive and gram-negative bacteria, their cytotoxicity to murine cells, and the biostability of the modified peptides in serum. We identified a novel antimicrobial peptide, WRK-30, with enhanced antimicrobial potency against S. aureus and MRSA. Additionally, WRK-30 was less cytotoxic to eukaryotic cells, allowing its application in higher concentrations in an in vivo setting. In conclusion, we identified a novel CLEC3A-derived antimicrobial peptide WRK-30 with significantly improved therapeutic properties and the potential to widen the repertoire of conventional antibiotics. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 12101532 2079-6382 |
| Relation: | https://www.mdpi.com/2079-6382/12/10/1532; https://doaj.org/toc/2079-6382 |
| DOI: | 10.3390/antibiotics12101532 |
| URL الوصول: | https://doaj.org/article/443eba95fde3488bb2fa481aff721d14 |
| رقم الأكسشن: | edsdoj.443eba95fde3488bb2fa481aff721d14 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 12101532 20796382 |
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| DOI: | 10.3390/antibiotics12101532 |