دورية أكاديمية
Nodulation Studies in the Model Legume Medicago truncatula: Advantages of Using the Constitutive EF1α Promoter and Limitations in Detecting Fluorescent Reporter Proteins in Nodule Tissues
| العنوان: | Nodulation Studies in the Model Legume Medicago truncatula: Advantages of Using the Constitutive EF1α Promoter and Limitations in Detecting Fluorescent Reporter Proteins in Nodule Tissues |
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| المؤلفون: | Marie-Christine Auriac, Antonius C. J. Timmers |
| المصدر: | Molecular Plant-Microbe Interactions, Vol 20, Iss 9, Pp 1040-1047 (2007) |
| بيانات النشر: | The American Phytopathological Society, 2007. |
| سنة النشر: | 2007 |
| المجموعة: | LCC:Microbiology LCC:Botany |
| مصطلحات موضوعية: | Sinorhizobium meliloti, Microbiology, QR1-502, Botany, QK1-989 |
| الوصف: | The Cauliflower mosaic virus 35S promoter currently is being used in RNAi-based approaches for attenuating host gene expression during legume root nodule development and also for the expression of fluorescent reporters in nodule tissues. In this study, we have evaluated the expression of this promoter in the indeterminate nodules of the model plant Medicago truncatula. Our results clearly show that the 35S promoter is inactive in both the nodule meristem and in bacteroid-containing cells of the nodules. On the other hand, the Arabidopsis thaliana EF1α promoter was found to be strongly expressed both in the nodule meristem and in all nodule-invaded cells. Therefore, we conclude that the constitutive EF1α promoter is far superior for mRNAi or overexpression studies in nodule tissues compared with the commonly used 35S promoter. In addition, our experiments have revealed that the intensity of fluorescent markers such as green fluorescent protein is severely attenuated within invaded cells in the nitrogen-fixation zone of the nodule, most likely by fluorescence quenching. This phenomenon may hinder the use of these tools for live-cell imaging in nodule tissue. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1943-7706 0894-0282 |
| Relation: | https://doaj.org/toc/0894-0282; https://doaj.org/toc/1943-7706 |
| DOI: | 10.1094/MPMI-20-9-1040 |
| URL الوصول: | https://doaj.org/article/45bae3a3eaa0405f96bf7e3e5f62daf9 |
| رقم الأكسشن: | edsdoj.45bae3a3eaa0405f96bf7e3e5f62daf9 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 19437706 08940282 |
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| DOI: | 10.1094/MPMI-20-9-1040 |