دورية أكاديمية
Protective effect of thymoquinone, the active constituent of Nigella sativa fixed oil, against ethanol toxicity in rats
| العنوان: | Protective effect of thymoquinone, the active constituent of Nigella sativa fixed oil, against ethanol toxicity in rats |
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| المؤلفون: | Sayed Masoud Hosseini, Elahe Taghiabadi, Khalil Abnous, Alireza Timcheh Hariri, Hamed Pourbakhsh, Hossein Hosseinzadeh |
| المصدر: | Iranian Journal of Basic Medical Sciences, Vol 20, Iss 8, Pp 927-939 (2017) |
| بيانات النشر: | Mashhad University of Medical Sciences, 2017. |
| سنة النشر: | 2017 |
| المجموعة: | LCC:Medicine |
| مصطلحات موضوعية: | Apoptosis, Ethanol toxicity, GSH content, Lipid peroxidation, Nigella sativa, Thymoquinone, Medicine |
| الوصف: | Objective(s): Long term consumption of ethanol may induce damage to many organs. Ethanol induces its noxious effects through reactive oxygen species production, and lipid peroxidation and apoptosis induction in different tissues and cell types. Previous experiments have indicated the antioxidant characteristics of thymoquinone, the active constituent of Nigella sativa fixed oil, against biologically dangerous reactive oxygen species. This experiment was planned to evaluate the protective effect of thymoquinone against subchronic ethanol toxicity in rats. Materials and Methods: Experiments were performed on six groups. Each group consisted of six animals, including control group (saline, gavage), ethanol-receiving group (3 g/kg/day, gavage), thymoquinone (2.5, 5, 10 mg/Kg/day, intraperitoneally (IP)) plus ethanol and thymoquinone (10 mg/Kg/day, IP) groups. Treatments were carried out in four weeks. Results: Thymoquinone reduced the ethanol-induced increase in the lipid peroxidation and severity of histopathological alteration in liver and kidney tissues. In addition it improved the levels of proinflammatory cytokines in liver tissue. Furthermore, thymoquinone corrected the liver enzymes level including alanine transaminase, aspartate transaminase and alkaline phosphatase in serum and glutathione content in liver and kidney tissues. Other experiments such as Western blot analysis and quantitative real-time RT-PCR revealed that thymoquinone suppressed the expression of Bax/Bcl-2 ratio (both protein and mRNA level), and caspases activation pursuant to ethanol toxicity. Conclusion: This study indicates that thymoquinone may have preventive effects against ethanol toxicity in the liver and kidney tissue through reduction in lipid peroxidation and inflammation, and also interrupting apoptosis. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2008-3866 2008-3874 |
| Relation: | http://ijbms.mums.ac.ir/article_9116_139a65e039c85160eb2ac12f07e7048a.pdf; https://doaj.org/toc/2008-3866; https://doaj.org/toc/2008-3874 |
| DOI: | 10.22038/ijbms.2017.9116 |
| URL الوصول: | https://doaj.org/article/48206637f6f34be787e33e652856dcd7 |
| رقم الأكسشن: | edsdoj.48206637f6f34be787e33e652856dcd7 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20083866 20083874 |
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| DOI: | 10.22038/ijbms.2017.9116 |