دورية أكاديمية
Fragmentation Through Polymerization (FTP): A new method to fragment DNA for next-generation sequencing.
| العنوان: | Fragmentation Through Polymerization (FTP): A new method to fragment DNA for next-generation sequencing. |
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| المؤلفون: | Konstantin B Ignatov, Konstantin A Blagodatskikh, Dmitry S Shcherbo, Tatiana V Kramarova, Yulia A Monakhova, Vladimir M Kramarov |
| المصدر: | PLoS ONE, Vol 14, Iss 4, p e0210374 (2019) |
| بيانات النشر: | Public Library of Science (PLoS), 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | Fragmentation of DNA is the very important first step in preparing nucleic acids for next-generation sequencing. Here we report a novel Fragmentation Through Polymerization (FTP) technique, which is a simple, robust, and low-cost enzymatic method of fragmentation. This method generates double-stranded DNA fragments that are suitable for direct use in NGS library construction and allows the elimination of the additional step of reparation of DNA ends. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1932-6203 |
| Relation: | https://doaj.org/toc/1932-6203 |
| DOI: | 10.1371/journal.pone.0210374 |
| URL الوصول: | https://doaj.org/article/48ba23100aec49d89fa39ae5c5748371 |
| رقم الأكسشن: | edsdoj.48ba23100aec49d89fa39ae5c5748371 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0210374 |