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Effect of DcR3-specific siRNA on cell growth suppression and apoptosis induction in glioma cells via affecting ERK and AKT

التفاصيل البيبلوغرافية
العنوان: Effect of DcR3-specific siRNA on cell growth suppression and apoptosis induction in glioma cells via affecting ERK and AKT
المؤلفون: Zhang Y, Huang SN, Leng YH, Chen X, Liu TT, Wang HL, Wei FL, Luo DZ, Chen G, Wei ZX
المصدر: OncoTargets and Therapy, Vol Volume 9, Pp 5195-5202 (2016)
بيانات النشر: Dove Medical Press, 2016.
سنة النشر: 2016
المجموعة: LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
مصطلحات موضوعية: DcR3-specific-siRNA, cell growth, apoptosis, glioma, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
الوصف: Yu Zhang,1,* Suning Huang,2,* Yuhua Leng,1 Xin Chen,1 Tiantian Liu,1 Hanlin Wang,1 Fanglin Wei,1 Dianzhong Luo,1 Gang Chen,1 Zhuxin Wei21Department of Pathology, 2Department of Radiotherapy, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, People’s Republic of China*These authors contributed equally to this work Background: Previously, we found that the expression of decoy receptor 3 (DcR3) in gliomas was significantly upregulated compared to normal brain tissues. However, the effect of DcR3-specific small interfering RNA (siRNA) on cell biological function of glioma cells remains incompletely understood.Objective: The aim of this study was to explore the effect of DcR3 siRNA on cell growth and apoptosis of glioma cells and to investigate the potential downstream pathways affected by DcR3.Methods: DcR3-specific siRNA was transfected into three glioma cell lines (U251MG, LN-308, and U87MG) using combiMAGnetofection method. MTS tetrazolium assay and fluorimetric resorufin viability assay were used to assess the growth of glioma cells. Then, apoptosis was examined using the Hoechst 33342/propidium iodide double-staining assay and fluorescent caspase-3/7 assay. Meanwhile, Western blot was performed to explore the probable pathway by which DcR3-specific siRNA acts in glioma cells. Also, microarray dataset analysis was applied to analyze the potential function of DcR3 in glioma.Results: The DcR3-specific siRNA had a potent effect on cell growth and apoptosis of all three glioma cells tested, and the effects were time dependent. Among these three glioma cell lines, U251MG had the most significant effect with regard to growth inhibition and apoptosis induction. MTS assay showed that the proliferation rate at 72 and 96 hours after the transfection was 76.333%±5.131% (t=7.611, P=0.002) and 64.333%±5.859% (t=10.983, P
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1178-6930
62154966
Relation: https://www.dovepress.com/effect-of-dcr3-specific-sirna-on-cell-growth-suppression-and-apoptosis-peer-reviewed-article-OTT; https://doaj.org/toc/1178-6930
URL الوصول: https://doaj.org/article/49385edf62154966b9232a14d51c813c
رقم الأكسشن: edsdoj.49385edf62154966b9232a14d51c813c
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:11786930
62154966