Efektifitas Penambahan Insulin dalam Media Maturasi dan atau Media Kultur pada Tingkat Maturasi Oosit dan Perkembangan Awal Embrio Sapi secara In Vitro
التفاصيل البيبلوغرافية
العنوان:
Efektifitas Penambahan Insulin dalam Media Maturasi dan atau Media Kultur pada Tingkat Maturasi Oosit dan Perkembangan Awal Embrio Sapi secara In Vitro
This study was aimed to determine the effect of insulin supplementation in maturation and/or culture medium on nuclear maturation rate and the early bovine embryo development in vitro. Oocytes were collected and matured in maturation media without (IVM I-) or with (IVM I+) 10 ug/µL insulin at incubator 5% CO2 and the temperature of 39 °C, for early embryonic development evaluation, oocytes were divided into 4 groups, without the supplementation of insulin to the maturation medium and culture (IVM I-/IVC I-), insulin supplementation only the maturation medium (IVM I+/IVC I-), insulin supplementation only in the culture medium (IVM I-/IVC I+), and the combination of insulin supplementation to the maturation medium and culture (IVM I+/IVC I+). The result showed that supplementation of insulin to the maturation medium increased number nuclear maturation was higher 87.7% (P
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