دورية أكاديمية
Knockdown of Vacuolar ATPase Subunit G Gene Affects Larval Survival and Impaired Pupation and Adult Emergence in Henosepilachna vigintioctopunctata
| العنوان: | Knockdown of Vacuolar ATPase Subunit G Gene Affects Larval Survival and Impaired Pupation and Adult Emergence in Henosepilachna vigintioctopunctata |
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| المؤلفون: | Jie Zeng, Wei-Nan Kang, Lin Jin, Ahmad Ali Anjum, Guo-Qing Li |
| المصدر: | Insects, Vol 12, Iss 10, p 935 (2021) |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Science |
| مصطلحات موضوعية: | Henosepilachna vigintioctopunctata, vacuolar ATPase subunit G, larval survival, gut, Malpighian tubule, Science |
| الوصف: | The vATPase holoenzyme consists of two functional subcomplexes, the cytoplasmic (peripheral) V1 and the membrane-embedded V0. Both V1 and V0 sectors contain eight subunits, with stoichiometry of A3B3CDE3FG3H in V1 and ac8c’c”def(Voa1p) in V0 respectively. However, the function of G subunit has not been characterized in any non-Drosophilid insect species. In the present paper, we uncovered that HvvATPaseG was actively transcribed from embryo to adult in a Coleopteran pest Henosepilachna vigintioctopunctata. Its mRNA levels peaked in larval hindgut and Malpighian tubules. RNA interference (RNAi)-mediated knockdown of HvvATPaseG significantly reduced larval feeding, affected chitin biosynthesis, destroyed midgut integrity, damaged midgut peritrophic membrane, and retarded larval growth. The function of Malpighian tubules was damaged, the contents of glucose, trehalose, lipid, total soluble amino acids and protein were lowered and the fat bodies were lessened in the HvvATPaseG RNAi larvae, compared with those in the PBS- and dsegfp-fed beetles. In contrast, the amount of glycogen was dramatically increased in the HvvATPaseG depletion ladybirds. As a result, the development was arrested, pupation was inhibited and adult emergence was impaired in the HvvATPaseG hypomorphs. Our results demonstrated that G subunit plays a critical role during larval development in H. vigintioctopunctata. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 12100935 2075-4450 |
| Relation: | https://www.mdpi.com/2075-4450/12/10/935; https://doaj.org/toc/2075-4450 |
| DOI: | 10.3390/insects12100935 |
| URL الوصول: | https://doaj.org/article/4e0367a44df14dae91924483bd260069 |
| رقم الأكسشن: | edsdoj.4e0367a44df14dae91924483bd260069 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 12100935 20754450 |
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| DOI: | 10.3390/insects12100935 |