دورية أكاديمية
Blueberry red ringspot virus eliminated from highbush blueberry by shoot tip culture
| العنوان: | Blueberry red ringspot virus eliminated from highbush blueberry by shoot tip culture |
|---|---|
| المؤلفون: | Josef ŠPAK, Daniela PAVINGEROVÁ, Jaroslava PŘIBYLOVÁ, Vlastimila ŠPAKOVÁ, František PAPRŠTEIN, Jiří SEDLÁK |
| المصدر: | Plant Protection Science, Vol 50, Iss 4, Pp 174-178 (2014) |
| بيانات النشر: | Czech Academy of Agricultural Sciences, 2014. |
| سنة النشر: | 2014 |
| المجموعة: | LCC:Plant culture |
| مصطلحات موضوعية: | brrv, in vitro, pcr, vaccinium corymbosum l., virus-free, virus elimination, Plant culture, SB1-1110 |
| الوصف: | In order to find an effective elimination method of Blueberry red ringspot virus (BRRV), shoot tips of 2-3 mm size originating from buds of highbush blueberry cv. Darrow naturally infected by BRRV and collected in winter months were regenerated and multiplied on woody-plant medium (WPM) with 1.5 mg/l zeatin and 20 g/l sucrose. Developed shoots tested negative by polymerase chain reaction (PCR) for BRRV were transferred onto a rooting medium consisting of WPM supplemented with 1 mg/l indole-3-butyric acid. Rooting plants were transferred into soil and tested twice by PCR 6 months apart. Ninety per cent eradication of BRRV was achieved while isolating shoot tips, multiplying them in one vegetative generation, and regenerating whole 50-60 cm high plants within 30 months. All plants developed rich fruits after their overwintering in a cold greenhouse and were tested BRRV negative in summer 2013. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1212-2580 1805-9341 |
| Relation: | https://pps.agriculturejournals.cz/artkey/pps-201404-0003_blueberry-red-ringspot-virus-eliminated-from-highbush-blueberry-by-shoot-tip-culture.php; https://doaj.org/toc/1212-2580; https://doaj.org/toc/1805-9341 |
| DOI: | 10.17221/84/2013-PPS |
| URL الوصول: | https://doaj.org/article/5324f5de33f644c2a4aa99462a210d11 |
| رقم الأكسشن: | edsdoj.5324f5de33f644c2a4aa99462a210d11 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 12122580 18059341 |
|---|---|
| DOI: | 10.17221/84/2013-PPS |