دورية أكاديمية
أعرض في EDS

Usefulness of modified Medium RD as a chemically defined medium for in vitro maturation of bovine oocytes

التفاصيل البيبلوغرافية
العنوان: Usefulness of modified Medium RD as a chemically defined medium for in vitro maturation of bovine oocytes
المؤلفون: Kenji Momozawa
المصدر: Reproductive Medicine and Biology, Vol 19, Iss 4, Pp 365-371 (2020)
بيانات النشر: Wiley, 2020.
سنة النشر: 2020
المجموعة: LCC:Diseases of the endocrine glands. Clinical endocrinology
LCC:Reproduction
مصطلحات موضوعية: bovine oocytes, defined medium, in vitro culture, in vitro maturation, medium RD, Diseases of the endocrine glands. Clinical endocrinology, RC648-665, Reproduction, QH471-489
الوصف: Abstract Purpose In the present study, I evaluated the usefulness of Medium RD, with mixed RPMI1640 and Dulbecco's modified Eagle's medium (1:1, v/v), as a chemically defined medium for in vitro maturation (IVM) of bovine oocytes. Methods In vitro maturation was performed in 10 mmol/L HEPES‐buffered TCM199 (mTCM199), 10 mmol/L HEPES‐buffered Medium RD (mRD), and mTCM199 supplemented with fetal bovine serum fraction (mTCM199 + FBS fraction) that served as control. Cumulus‐oocyte complexes were matured for 24 hours in three different media supplemented with follicle‐stimulating hormone, estradiol‐17β, and polyvinylpyrrolidone. Nuclear maturation of oocytes, their developmental competence into blastocysts after in vitro fertilization (IVF) and mitochondrial distribution in oocytes were investigated. Results There was no difference in the ratio of matured oocytes regardless of IVM media. The percentage of morula stage was higher in mRD than in mTCM199 group (P
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1447-0578
1445-5781
Relation: https://doaj.org/toc/1445-5781; https://doaj.org/toc/1447-0578
DOI: 10.1002/rmb2.12337
URL الوصول: https://doaj.org/article/585bb1a3969049e88477d97a903e9ecf
رقم الأكسشن: edsdoj.585bb1a3969049e88477d97a903e9ecf
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:14470578
14455781
DOI:10.1002/rmb2.12337