دورية أكاديمية
Molecular techniques for the study and diagnosis of parasite infection
| العنوان: | Molecular techniques for the study and diagnosis of parasite infection |
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| المؤلفون: | RG Tavares, R Staggemeier, ALP Borges, MT Rodrigues, LA Castelan, J Vasconcelos, ME Anschau, SM Spalding |
| المصدر: | Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 17, Iss 3, Pp 239-248 (2011) |
| بيانات النشر: | SciELO, 2011. |
| سنة النشر: | 2011 |
| المجموعة: | LCC:Arctic medicine. Tropical medicine LCC:Toxicology. Poisons LCC:Zoology |
| مصطلحات موضوعية: | parasite infection, diagnosis, molecular techniques, molecular epidemiology, Arctic medicine. Tropical medicine, RC955-962, Toxicology. Poisons, RA1190-1270, Zoology, QL1-991 |
| الوصف: | In parasitology, routine laboratory diagnosis involves conventional methods, such as optical microscopy, used for the morphological identification of parasites. Currently, molecular biology techniques are increasingly used to diagnose parasite structures in order to enhance the identification and characterization of parasites. The objective of the present study was to review the main current and new diagnostic techniques for confirmation of parasite infections, namely: polymerase chain reaction (PCR), real-time polymerase chain reaction (RT-PCR), loop-mediated isothermal amplification (LAMP), Luminex xMAP, random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and restriction fragment length polymorphism (RFLP), in addition to microsatellites. Molecular assays have comprehensively assisted in the diagnosis, treatment and epidemiological studies of parasitic diseases that affect people worldwide, helping to control parasitic disease mortality. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1678-9199 57295794 |
| Relation: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300003; https://doaj.org/toc/1678-9199 |
| DOI: | 10.1590/S1678-91992011000300003 |
| URL الوصول: | https://doaj.org/article/624da572957945138236a40946f67fc6 |
| رقم الأكسشن: | edsdoj.624da572957945138236a40946f67fc6 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 16789199 57295794 |
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| DOI: | 10.1590/S1678-91992011000300003 |