دورية أكاديمية
Detection of Staphylococcus aureus in food by droplet digital polymerase chain reaction
| العنوان: | Detection of Staphylococcus aureus in food by droplet digital polymerase chain reaction |
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| المؤلفون: | LI Dan, XU Leirui, WEI Haiyan, WEI Yongxin, MA Dan, WANG Qi, ZHANG Ximeng, ZHANG Feng, ZENG Jing |
| المصدر: | Zhongguo shipin weisheng zazhi, Vol 33, Iss 03, Pp 284-290 (2021) |
| بيانات النشر: | The Editorial Office of Chinese Journal of Food Hygiene, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Food processing and manufacture LCC:Nutrition. Foods and food supply |
| مصطلحات موضوعية: | droplet digital polymerase chain reaction, staphylococcus aureus, quantitative detection, Food processing and manufacture, TP368-456, Nutrition. Foods and food supply, TX341-641 |
| الوصف: | ObjectiveTo establish a new quantitative method for detection of Staphylococcus aureus in food by droplet digital polymerase chain reaction (ddPCR).MethodsA pair of primers and probe for both qPCR and ddPCR was selected according to the single copy gene of nuc in Staphylococcus aureus. The specificity, sensitivity, accuracy and repeatability of this method were analyzed.ResultsThe limit of detection (LOD) and limit of quantitation (LOQ) of ddPCR method were determined to be 110 CFU/mL by gradient dilution of pure culture of Staphylococcus aureus. The LOQ of ddPCR was 1 000 CFU/g in the artificial contaminated experiment, and the maximum deviation of detection result of four spiked levels was 4.77%, and the coefficient of variation of 5 parallel repeats was less than 20%, showing good accuracy and repeatability.ConclusionThe established method has good specificity, high sensitivity and accurate result, which provides a reference for rapid quantitative detection of Staphylococcus aureus. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | Chinese |
| تدمد: | 1004-8456 |
| Relation: | http://www.zgspws.com/zgspwszz/article/abstract/20210308?st=article_issue; https://doaj.org/toc/1004-8456 |
| DOI: | 10.13590/j.cjfh.2021.03.008 |
| URL الوصول: | https://doaj.org/article/69c62a3e4f6f4bbd8dfc0d9d426f1893 |
| رقم الأكسشن: | edsdoj.69c62a3e4f6f4bbd8dfc0d9d426f1893 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 10048456 |
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| DOI: | 10.13590/j.cjfh.2021.03.008 |