دورية أكاديمية
Deeper exploration of inflammatory cell populations in milk to monitor udder health in dairy cows
| العنوان: | Deeper exploration of inflammatory cell populations in milk to monitor udder health in dairy cows |
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| المؤلفون: | Carolina Menezes Suassuna de Souza, Maiara Garcia Blagitz, Fernando Nogueira Souza, Camila Freitas Batista, Daniel Magalhães Lima, Alexandre José Alves, Marcos Bryan Heinemann, Eduardo Milton Ramos Sanchez, Hugo Frias Torres, Artur Cezar de Carvalho Fernandes, Alice Maria Melville Paiva Della Libera |
| المصدر: | Scientia Agricola, Vol 80 (2023) |
| بيانات النشر: | Universidade de São Paulo, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Agriculture (General) |
| مصطلحات موضوعية: | differential cell count, somatic cell count, diagnosis, mastitis, dairy cattle, Agriculture (General), S1-972 |
| الوصف: | ABSTRACT The present study explored the predictive values of milk leukocyte differentials (MLD) as a basis for improving the diagnosis of intramammary infections (IMIs) and subclinical mastitis. Quarter milk samples were collected for bacteriological analysis, quarter somatic cell count (qSCC), and MLD. The MLD were assessed using the cytospin technique, direct microscopic smears, and flow cytometry. The predictive values of each single leukocyte population and useful potential indices that could better reflect immune complexity were also calculated. Changes in the percentage of any leukocyte alone failed to substantially improve the predictive value of qSCC in diagnosing IMIs. Although certain parameters increased the area under the receiver operating characteristic curve (ROC curve) as a result of increased specificity values, a slight reduction in sensitivity was observed. The so-called CD8 complex was a unique parameter which improved both the sensitivity (78.79 %) and the specificity (80.77 %) in IMI diagnosis, resulting in the highest area under the ROC curve (0.87). To diagnose subclinical mastitis, the percentage of macrophages and the sum of the percentage PMNLs and T CD8+ cells divided by the percentage of macrophages showed the highest predictive values (sensitivity = 79.63, specificity = 73.68, and area under the ROC curve = 0.83) in the differentiation of the inflammatory condition status of cows. In conclusion, this study provides further insights into using T CD8+ lymphocytes in diagnosing bovine IMIs, combined with PMNLs and macrophages. The antidromic trend of macrophages vs. PMNLs and T CD8+ lymphocytes due to the increasing qSCCs was crucial to differentiating quarters under both inflammatory and non-inflammatory conditions. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Spanish; Castilian Portuguese |
| تدمد: | 1678-992X 1678-992x |
| Relation: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162023000100408&tlng=en; https://doaj.org/toc/1678-992X |
| DOI: | 10.1590/1678-992x-2022-0086 |
| URL الوصول: | https://doaj.org/article/6a48d9e16cc9404490a6bcd8162122c6 |
| رقم الأكسشن: | edsdoj.6a48d9e16cc9404490a6bcd8162122c6 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 1678992X 1678992x |
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| DOI: | 10.1590/1678-992x-2022-0086 |