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Spatiotemporal Expression of Anticoagulation Factor Antistasin in Freshwater Leeches

التفاصيل البيبلوغرافية
العنوان: Spatiotemporal Expression of Anticoagulation Factor Antistasin in Freshwater Leeches
المؤلفون: Hee-Jin Kwak, Jeong-Su Park, Brenda Irene Medina Jiménez, Soon Cheol Park, Sung-Jin Cho
المصدر: International Journal of Molecular Sciences, Vol 20, Iss 16, p 3994 (2019)
بيانات النشر: MDPI AG, 2019.
سنة النشر: 2019
المجموعة: LCC:Biology (General)
LCC:Chemistry
مصطلحات موضوعية: Antistasin, Helobdella, in situ hybridization, expression, hedgehog signaling, Biology (General), QH301-705.5, Chemistry, QD1-999
الوصف: Antistasin, which was originally discovered in the salivary glands of the Mexican leech Haementeria officinalis, was newly isolated from Helobdella austinensis. To confirm the temporal expression of antistasin during embryogenesis, we carried out semi-quantitative RT-PCR. Hau-antistasin1 was uniquely expressed at stage 4 of the cleavage and was strongly expressed in the late stages of organogenesis, as were other antistasin members. In order to confirm the spatial expression of antistasin, we performed fluorescence in situ hybridization in the late stages of organogenesis. The expression of each antistasin in the proboscis showed a similar pattern and varied in expression in the body. In addition, the spatial expression of antistasin orthologs in different leeches showed the possibility of different function across leech species. Hau-antistasin1 was expressed in the same region as hedgehog, which is a known mediator of signal transduction pathway. Hau-antistasin1 is probably a downstream target of Hedgehog signaling, involved in segment polarity signal pathway.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1422-0067
Relation: https://www.mdpi.com/1422-0067/20/16/3994; https://doaj.org/toc/1422-0067
DOI: 10.3390/ijms20163994
URL الوصول: https://doaj.org/article/decac6a8934143f487094fa0b7fa1772
رقم الأكسشن: edsdoj.6a8934143f487094fa0b7fa1772
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:14220067
DOI:10.3390/ijms20163994