دورية أكاديمية
Design and 3D printing of an electrochemical sensor for Listeria monocytogenes detection based on loop mediated isothermal amplification
| العنوان: | Design and 3D printing of an electrochemical sensor for Listeria monocytogenes detection based on loop mediated isothermal amplification |
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| المؤلفون: | Ane Rivas-Macho, Unai Eletxigerra, Ruth Diez-Ahedo, Santos Merino, Antton Sanjuan, M. Mounir Bou-Ali, Leire Ruiz-Rubio, Javier del Campo, José Luis Vilas-Vilela, Felipe Goñi-de-Cerio, Garbiñe Olabarria |
| المصدر: | Heliyon, Vol 9, Iss 1, Pp e12637- (2023) |
| بيانات النشر: | Elsevier, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Science (General) LCC:Social sciences (General) |
| مصطلحات موضوعية: | Loop-mediated isothermal AMPlification (LAMP), Listeria monocytogenes, Electrochemical detection, Biosensor, 3D printing, Numerical analysis, Science (General), Q1-390, Social sciences (General), H1-99 |
| الوصف: | The aim of this work is the design and 3D printing of a new electrochemical sensor for the detection of Listeria monocytogenes based on loop mediated isothermal amplification (LAMP). The food related diseases involve a serious health issue all over the world. Listeria monocytogenes is one of the major problems of contaminated food, this pathogen causes a disease called listeriosis with a high rate of hospitalization and mortality. Having a fast, sensitive and specific detection method for food quality control is a must in the food industry to avoid the presence of this pathogen in the food chain (raw materials, facilities and products). A point-of-care biosensor based in LAMP and electrochemical detection is one of the best options to detect the bacteria on site and in a very short period of time. With the numerical analysis of different geometries and flow rates during sample injection in order to avoid bubbles, an optimized design of the microfluidic biosensor chamber was selected for 3D-printing and experimental analysis.For the electrochemical detection, a novel custom gold concentric-3-electrode consisting in a working electrode, reference electrode and a counter electrode was designed and placed in the bottom of the chamber. The LAMP reaction was optimized specifically for a primers set with a limit of detection of 1.25 pg of genomic DNA per reaction and 100% specific for detecting all 12 Listeria monocytogenes serotypes and no other Listeria species or food-related bacteria. The methylene blue redox-active molecule was tested as the electrochemical transducer and shown to be compatible with the LAMP reaction and very clearly distinguished negative from positive food samples when the reaction is measured at the end-point inside the biosensor. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2405-8440 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2405844022039251; https://doaj.org/toc/2405-8440 |
| DOI: | 10.1016/j.heliyon.2022.e12637 |
| URL الوصول: | https://doaj.org/article/6b0f06832bcf4e77b03ae8f19fff1978 |
| رقم الأكسشن: | edsdoj.6b0f06832bcf4e77b03ae8f19fff1978 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 24058440 |
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| DOI: | 10.1016/j.heliyon.2022.e12637 |