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An immuno-wall microdevice exhibits rapid and sensitive detection of IDH1-R132H mutation specific to grade II and III gliomas

التفاصيل البيبلوغرافية
العنوان: An immuno-wall microdevice exhibits rapid and sensitive detection of IDH1-R132H mutation specific to grade II and III gliomas
المؤلفون: Akane Yamamichi, Toshihiro Kasama, Fumiharu Ohka, Hiromichi Suzuki, Akira Kato, Kazuya Motomura, Masaki Hirano, Melissa Ranjit, Lushun Chalise, Michihiro Kurimoto, Goro Kondo, Kosuke Aoki, Noritada Kaji, Manabu Tokeshi, Toshio Matsubara, Takeshi Senga, Mika K. Kaneko, Hidenori Suzuki, Masahito Hara, Toshihiko Wakabayashi, Yoshinobu Baba, Yukinari Kato, Atsushi Natsume
المصدر: Science and Technology of Advanced Materials, Vol 17, Iss 1, Pp 618-625 (2016)
بيانات النشر: Taylor & Francis Group, 2016.
سنة النشر: 2016
المجموعة: LCC:Materials of engineering and construction. Mechanics of materials
LCC:Biotechnology
مصطلحات موضوعية: glioma, isocitrate dehydrogenase 1 mutation, immuno-wall microdevice, rapid diagnosis, precision medicine, Materials of engineering and construction. Mechanics of materials, TA401-492, Biotechnology, TP248.13-248.65
الوصف: World Health Organization grade II and III gliomas most frequently occur in the central nervous system (CNS) in adults. Gliomas are not circumscribed; tumor edges are irregular and consist of tumor cells, normal brain tissue, and hyperplastic reactive glial cells. Therefore, the tumors are not fully resectable, resulting in recurrence, malignant progression, and eventual death. Approximately 69–80% of grade II and III gliomas harbor mutations in the isocitrate dehydrogenase 1 gene (IDH1), of which 83–90% are found to be the IDH1-R132H mutation. Detection of the IDH1-R132H mutation should help in the differential diagnosis of grade II and III gliomas from other types of CNS tumors and help determine the boundary between the tumor and normal brain tissue. In this study, we established a highly sensitive antibody-based device, referred to as the immuno-wall, to detect the IDH1-R132H mutation in gliomas. The immuno-wall causes an immunoreaction in microchannels fabricated using a photo-polymerizing polymer. This microdevice enables the analysis of the IDH1 status with a small sample within 15 min with substantially high sensitivity. Our results suggested that 10% content of the IDH1-R132H mutation in a sample of 0.33 μl volume, with 500 ng protein, or from 500 cells is theoretically sufficient for the analysis. The immuno-wall device will enable the rapid and highly sensitive detection of the IDH1-R132H mutation in routine clinical practice.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1468-6996
1878-5514
14686996
Relation: https://doaj.org/toc/1468-6996; https://doaj.org/toc/1878-5514
DOI: 10.1080/14686996.2016.1227222
URL الوصول: https://doaj.org/article/6c440a375bf246eaa8f3b8f1444d834d
رقم الأكسشن: edsdoj.6c440a375bf246eaa8f3b8f1444d834d
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:14686996
18785514
DOI:10.1080/14686996.2016.1227222