دورية أكاديمية
Gellan Gum Promotes the Differentiation of Enterocytes from Human Induced Pluripotent Stem Cells
| العنوان: | Gellan Gum Promotes the Differentiation of Enterocytes from Human Induced Pluripotent Stem Cells |
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| المؤلفون: | Shimeng Qiu, Tomoki Kabeya, Isamu Ogawa, Shiho Anno, Hisato Hayashi, Tatsuro Kanaki, Tadahiro Hashita, Takahiro Iwao, Tamihide Matsunaga |
| المصدر: | Pharmaceutics, Vol 12, Iss 10, p 951 (2020) |
| بيانات النشر: | MDPI AG, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Pharmacy and materia medica |
| مصطلحات موضوعية: | human induced pluripotent stem cells, gellan gum, enterocytes, drug transporter, drug development, Pharmacy and materia medica, RS1-441 |
| الوصف: | The evaluation of drug pharmacokinetics in the small intestine is critical for developing orally administered drugs. Caucasian colon adenocarcinoma (Caco-2) cells are employed to evaluate drug absorption in preclinical trials of drug development. However, the pharmacokinetic characteristics of Caco-2 cells are different from those of the normal human small intestine. Besides this, it is almost impossible to obtain primary human intestinal epithelial cells of the same batch. Therefore, human iPS cell-derived enterocytes (hiPSEs) with pharmacokinetic functions similar to human intestinal epithelial cells are expected to be useful for the evaluation of drug absorption. Previous studies have been limited to the use of cytokines and small molecules to generate hiPSEs. Dietary fibers play a critical role in maintaining intestinal physiology. We used gellan gum (GG), a soluble dietary fiber, to optimize hiPSE differentiation. hiPSEs cocultured with GG had significantly higher expression of small intestine- and pharmacokinetics-related genes and proteins. The activities of drug-metabolizing enzymes, such as cytochrome P450 2C19, and peptide transporter 1 were significantly increased in the GG treatment group compared to the control group. At the end point of differentiation, the percentage of senescent cells increased. Therefore, GG could improve the differentiation efficiency of human iPS cells to enterocytes and increase intestinal maturation by extending the life span of hiPSEs. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 12100951 1999-4923 |
| Relation: | https://www.mdpi.com/1999-4923/12/10/951; https://doaj.org/toc/1999-4923 |
| DOI: | 10.3390/pharmaceutics12100951 |
| URL الوصول: | https://doaj.org/article/6cc496b6a04f43cb9e6e94f363fe0ea1 |
| رقم الأكسشن: | edsdoj.6cc496b6a04f43cb9e6e94f363fe0ea1 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 12100951 19994923 |
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| DOI: | 10.3390/pharmaceutics12100951 |