دورية أكاديمية
Epithelial and Mesenchymal-like Pancreatic Cancer Cells Exhibit Different Stem Cell Phenotypes Associated with Different Metastatic Propensities
| العنوان: | Epithelial and Mesenchymal-like Pancreatic Cancer Cells Exhibit Different Stem Cell Phenotypes Associated with Different Metastatic Propensities |
|---|---|
| المؤلفون: | Lisa-Marie Philipp, Umut-Ulas Yesilyurt, Arne Surrow, Axel Künstner, Anne-Sophie Mehdorn, Charlotte Hauser, Jan-Paul Gundlach, Olga Will, Patrick Hoffmann, Lea Stahmer, Sören Franzenburg, Hendrike Knaack, Udo Schumacher, Hauke Busch, Susanne Sebens |
| المصدر: | Cancers, Vol 16, Iss 4, p 686 (2024) |
| بيانات النشر: | MDPI AG, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens |
| مصطلحات موضوعية: | pancreatic adenocarcinoma, PDAC, cancer stem cells, EMT, epithelial–mesenchymal-transition, heterogeneity, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282 |
| الوصف: | Pancreatic ductal adenocarcinoma (PDAC) is mostly diagnosed at advanced or even metastasized stages, limiting the prognoses of patients. Metastasis requires high tumor cell plasticity, implying phenotypic switching in response to changing environments. Here, epithelial–mesenchymal transition (EMT), being associated with an increase in cancer stem cell (CSC) properties, and its reversion are important. Since it is poorly understood whether different CSC phenotypes exist along the EMT axis and how these impact malignancy-associated properties, we aimed to characterize CSC populations of epithelial and mesenchymal-like PDAC cells. Single-cell cloning revealed CSC (Holoclone) and non-CSC (Paraclone) clones from the PDAC cell lines Panc1 and Panc89. The Panc1 Holoclone cells showed a mesenchymal-like phenotype, dominated by a high expression of the stemness marker Nestin, while the Panc89 Holoclone cells exhibited a SOX2-dominated epithelial phenotype. The Panc89 Holoclone cells showed enhanced cell growth and a self-renewal capacity but slow cluster-like invasion. Contrarily, the Panc1 Holoclone cells showed slower cell growth and self-renewal ability but were highly invasive. Moreover, cell variants differentially responded to chemotherapy. In vivo, the Panc1 and Panc89 cell variants significantly differed regarding the number and size of metastases, as well as organ manifestation, leading to different survival outcomes. Overall, these data support the existence of different CSC phenotypes along the EMT axis in PDAC, manifesting different metastatic propensities. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 16040686 2072-6694 |
| Relation: | https://www.mdpi.com/2072-6694/16/4/686; https://doaj.org/toc/2072-6694 |
| DOI: | 10.3390/cancers16040686 |
| URL الوصول: | https://doaj.org/article/e6cf02b7fd6944b09cfde7ae0edb9f12 |
| رقم الأكسشن: | edsdoj.6cf02b7fd6944b09cfde7ae0edb9f12 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 16040686 20726694 |
|---|---|
| DOI: | 10.3390/cancers16040686 |